Abstract Flue-cured tobacco brown spot disease is a main fungal disease in the ripening stage of tobacco leaves caused by Alternaria alternata, which can cause huge economic loss in the field period and baking period. Endophytic and phyllosphere fungi are closely related to the happening of such disease. In this study, the PCR technology was used to amplify ITS1 region of all the fungal transcribed spacer regions of the flue-cured leaves of healthy and diseased plants, and then the leaf fungal community structure and diversity were analyzed using Illumina Miseq high-throughput sequencing technology. The result showed that Alternaria was dominant genus in all diseased tobacco leaves, and the relative abundance of Alternaria in each sample was more than 70%. Rhodotorula was second dominant genus in all diseased tobacco leaves, and the relative abundance in each sample was more than 10%. The relative abundances of other genera of fungi were less than 1% in all diseased leaves. For healthy tobacco leaves, Alternaria and Rhodotorula were the dominant genera, the sum of relative abundances of the two was smaller than that in diseased tobacco leaves. In addition to the above two species, the relative abundance of Aspergillus, Wallemia and Unclassified_o_Pleosporales, etc., were also greater than 1%. The results of alpha diversity analysis showed that the richness and diversity index of the fungal community in the diseased leaves were lower than that in the healthy leaves. Alternaria and Rhodotorula were the dominant genera in both healthy and diseased flue-cured tobacco leaves.
XIANG Ligang, WANG Hancheng, ZHENG Ping, CAI Liuti, YU Zhihe. Analysis of endophytic fungi and phyllosphere fungi of flue cured tobacco leaves with brown spot disease[J]. Acta Tabacaria Sinica, 2020, 26(4): 93-100.