Abstract [Objective] This study aims to screen out high-quality air-cured tobacco materials.[Methods] 35 air-cured tobacco germplasms were planted in Yanhe Town, Yuxi City, Yunnan Province. After air curing, middle leaves collected selected as research objects to study routine chemical components as well as contents of polyphenol, petroleum ether extracts, polyacid polybasic, higher fatty acid, and some aroma components using correlation analysis and cluster analysis.[Results] The content ranges of basic components (and the highest value type) were nitrogen 1.62% to 3.44% (air-cured), nicotine 2.30% to 9.76% (air-cured), potassium 0.73% to 3.42% (Nicotiana rustica L), polyphenol 1.51% to 6.43% (air-cured), petroleum ether extracts 2.66%~8.94% (cigar), and oxalic acid 7.09 to 50.21mg/g (aircured), malic acid 41.97 to 173.39mg/g (Nicotiana rustica L), and citric acid 16.02 to 126.45mg/g (Nicotiana rustica L), etc. The 35 aircured tobacco germplasms can be grouped into five categories:including type I, the Nicotiana rustica L, characterized by high potassium content, high malic acid and high citric acid, and with low sugar, polyphenol, petroleum ether extracts, oxalic acid and malonic acid; Type II, including the germplasm of Maryland tobacco, air-cured and cigar, characterized by high petroleum ether extracts, higher malic acid and citric acid; Type III, the air-cured tobacco germplasms, characterized by high polyphenol; Type IV characterized by high nitrogen and nicotine, higher oxalic acid; Type V characterized by high with total sugar, return sugar and starch, with high two sugar difference, sugaralkali ratio, nitrogen-base ratio, and low with malic acid, citric acid.