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中国烟草学报
Acta Tabacaria Sinica  2020, Vol. 26 Issue (4): 26-34    DOI: 10.16472/j.chinatobacco.2019.369
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Effects of air-curing humidity on key enzyme activities related to carbon and nitrogen metabolism and quality of cigar tobacco leaves
LU Shaohao1, ZHANG Jiawen1, ZHAO Zhe1, ZHONG Qiu2, WANG Jun2, SONG Zhaopeng1, ZOU Yuhang2, ZHANG Huashu2, ZHAO Mingqin1
1 College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China;
2 Deyang Tobacco Company of Sichuan Province, Deyang 618400, China
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Abstract In order to regulate the level of carbon and nitrogen metabolism during airing of tobacco leave and improve the quality of cigar tobacco leaves, the changes of water content, chemical composition and the activity of key enzymes related to carbon and nitrogen metabolism in cigar tobacco leaves were studied by using Dexue No.1 as the test material. The results showed that the changes of the chemical composition and the activity of key enzymes related to carbon and nitrogen metabolism in tobacco leaves were basically the same under different air curing humidity. The contents of water, total sugar, reducing sugar, starch, total nitrogen, protein and plastid pigment showed a downward trend, but the contents of amino acid and nicotine increased first and then decreased. The activity of amylase(AL) and neutral invertase(NI) showed a single peak curve, but the time to peak was slightly different. The activities of nitrate reductase (NR) and glutamine synthetase (GS) showed a downward trend. During air curing of cigar tobacco leaves, when the humidity was controlled within the range of 80%-85% in withering period, 75%-80% in yellowing period, 70%-75% in browning period, 65%-70% in fixing color period and 45%-50% in dry gluten period, the activities of AL, NI, NR and GS were the highest, the chemical components were suitable and the overall physical characteristics and quality were good. Therefore, controlling the air curing humidity within a certain range can improve the carbon and nitrogen metabolism of ciar tobacco leaves, promote conversion of tobacco leaf contents, and improve the quality and availability of cigar tobacco leaves.
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Articles by authors
LU Shaohao
ZHANG Jiawen
ZHAO Zhe
ZHONG Qiu
WANG Jun
SONG Zhaopeng
ZOU Yuhang
ZHANG Huashu
ZHAO Mingqin
Key words:  air curing humidity  cigar tobacco leaf  carbon and nitrogen metabolism  enzyme activity  chemical composition  sensory quality    
Received:  2019-11-07      Published::  2020-09-18
Cite this article:    
LU Shaohao, ZHANG Jiawen, ZHAO Zhe, ZHONG Qiu, WANG Jun, SONG Zhaopeng, ZOU Yuhang, ZHANG Huashu, ZHAO Mingqin. Effects of air-curing humidity on key enzyme activities related to carbon and nitrogen metabolism and quality of cigar tobacco leaves[J]. Acta Tabacaria Sinica, 2020, 26(4): 26-34.
URL:  
http://ycxb.tobacco.org.cn/EN/10.16472/j.chinatobacco.2019.369  or          http://ycxb.tobacco.org.cn/EN/Y2020/V26/I4/26
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