Nicotine conversion rate of 16 cigar varieties and its relationship with TSNAs content in fermented tobacco leaves
ZHOU Di1, SUN Yuqi1, SHI Hongzhi1, YANG Xingyou2,3, ZHOU Jun4, LU Ximei1, BAI Ruoshi4, YANG Weili2, ZHAO Yuanyuan1, QIN Yanqing3
1 National Tobacco Cultivation&Physiology&Biochemistry Research Center, Tobacco Cultivation Key Laboratory of China Tobacco, Tobacco Harm Reduction Research Center Zhengzhou 450002, China; 2 Dazhou Municipal Tobacco Company, Dazhou 635000, Sichuan, China; 3 Sichuan Provincial Tobacco Company, Chengdu 600041, China; 4 Shanghai Tobacco Group Beijing Cigarette Factory, Tongzhou District, Beijing 100024, China
Abstract [Objective] This study aims to investigate the difference in nicotine conversion rate among different cigar varieties as well as the relationship between nicotine conversion rate and TSNAs content in cigar tobacco. [Methods] The alkaloids, nitrate and TSNAs contents in the middle leaves of 16 cigar varieties were analyzed. [Results] 1） All cigar varieties had the problem of nicotine to nornicotine conversion at different degrees. The nicotine contents of 16 cigar varieties were in the range of 1.14% to 5.42%, averaging at 3.02%; and nornicotine contents changed in the range from 0.11% to 1.47%, averaging at 0.40%. The nicotine conversion rate ranged from 5.03% to 53.12%, with the average value of 12.66%, and the variation coefficient of nicotine conversion among varieties was as high as 101.01%. 2) The average nornicotine content and nicotine conversion rate of cigar wrapper varieties were significantly higher than those of cigar filler varieties. 3) The content and proportion of TSNAs in tobacco leaves of different cigar tobacco varieties were significantly different. The variation range of total TSNAs content was 181.74~7759.1 ng/g, with an average of 3010.98 ng/g. The content of NNN was the highest, with the average value of 1616.59 ng/g, and the variability was also the highest, with the coefficient of variation as high as 97.28%. 4） Correlation analysis showed that there was a significant positive correlation between nornicotine content and NNN and TSNAs content in 16 cigar tobacco varieties, with the correlation coefficient between NNN and nornicotine content reaching 0.802. [Conclusion] The high level of nornicotine content resulted from nicotine conversion is one of the main cause for the increased formation and accumulation of NNN and total TSNAs in cigar tobacco leaves.
ZHOU Di, SUN Yuqi, SHI Hongzhi, YANG Xingyou, ZHOU Jun, LU Ximei, BAI Ruoshi, YANG Weili, ZHAO Yuanyuan, QIN Yanqing. Nicotine conversion rate of 16 cigar varieties and its relationship with TSNAs content in fermented tobacco leaves[J]. Acta Tabacaria Sinica, 2020, 26(4): 18-25.