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Acta Tabacaria Sinica
ZHONGGUO YANCAO XUEBAO
Bimonthly
Inaugurated in 1992

Superintended by:
China Association for Science and Technology
Sponsored by:
Chinese Tobacco Society
Editor-in-Chief: YUAN Xingsi
Deputy Editor-in-Chief:
Editors: HA Junli, LIU Xinhua,
             AI Jitao, ZHANG Zheng
English Proofread by:
ZHAO Baidong, XIAO Boxue
Published by:
Editorial Office of Acta Tabacaria Sinica
Address: No.55 Yuetan South Street, Xicheng District, Beijing
Post Code: 100045
Tel: (86-10)63605768, 63605021, 63606237, 63605769, 63606007
Fax: (86-10)63605760
Email: xh-bj@tobacco.gov.cn
ISSN 1004-5708
CN 11-2985/TS

  Editor’s Pick More>>   
Current Issue Full Abstracts Archive
2020  Vol. 26 No. 6    Published: 28 December 2020
TOBACCO AND SMOKE CHEMISTRY    MANUFACTURING TECHNOLOGY    TOBACCO EQUIPMENT    AGRONOMY AND CURING    MODERN TOBACCO AGRICULTURE    ECONOMY AND MANAGEMENT       
 TOBACCO AND SMOKE CHEMISTRY
WU Shuaifan, CUI Xiuming, HUANG Yan
Study on the interaction of nicotine and benzoic acid
2020 Vol. 26 (6): 1-5 [Abstract] ( 14 ) HTML (0 KB)  PDF-CHN (1209 KB)  PDF-ENG  (0 KB)  ( 33 ) | Cite this article
ZHAO Guoling, YIN Xinqiang, HUANG Jianguo, GONG Shuguo, DAI Yuangang, ZHONG Kejun, PENG Xinhui
Study on the release of nicotine in different forms in e-cigarette aerosol
2020 Vol. 26 (6): 6-10 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (908 KB)  PDF-ENG  (0 KB)  ( 25 ) | Cite this article
ZHAO Deqing, DENG Yong, GUO Linqing, SHI Jianyang, WANG Shuaipeng, LIANG Kun, HAN Donglin, HUANG Yuchuan
Rapid determination of glycerol in HTP cigarette with enzyme electrode method
2020 Vol. 26 (6): 11-17 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (1242 KB)  PDF-ENG  (0 KB)  ( 16 ) | Cite this article
LI Bo, ZHANG Shuming, SONG Shihao, WANG Mingxia, LIU Zhen, WANG Hui, ZHANG Congyue, YUE Mingfei, YIN Shaokai
Sensory evaluation of mixed smoke of traditional cigarette and electronic cigarette: Research on transboundary electronic cigarette based on fuzzy mathematics
2020 Vol. 26 (6): 18-24 [Abstract] ( 5 ) HTML (0 KB)  PDF-CHN (1477 KB)  PDF-ENG  (0 KB)  ( 18 ) | Cite this article
LIU Xiucai, LAN Hongqiao, LIN Yan, LI Qiaoling, LIAN Fenyan, ZHENG Quanxing, YE Zhongli, ZHONG Tinggui, SU Mingliang
Distribution and transfer of menthol in mentholated cigarette with breakable capsule in filter
2020 Vol. 26 (6): 25-31 [Abstract] ( 10 ) HTML (0 KB)  PDF-CHN (1182 KB)  PDF-ENG  (0 KB)  ( 14 ) | Cite this article
 MANUFACTURING TECHNOLOGY
HE Wei, FAN Jianqiang, MU Wenjun, CHEN Yiqiang, BAO Kexiang, LIN Jian, ZHANG mingqian, ZHANG Ruiqiang, LIU Jingchuan, SONG Jizhen, WANG Chunlu
Construction of green aging technology system for flue-cured tobacco strips using four-stage controlled atmosphere method
2020 Vol. 26 (6): 32-44 [Abstract] ( 5 ) HTML (0 KB)  PDF-CHN (3149 KB)  PDF-ENG  (0 KB)  ( 14 ) | Cite this article
GAO Zhanyong, WANG Hui, YANG Jingjin, LU Honglin, WANG Yuzhen, HUA Yikun, LI Siyuan, YANG Jiadong, GAO Hui
Chromatic value of different segments of main vein of flue-cured tobacco and analysis by Fisher optimal segmentation method
2020 Vol. 26 (6): 45-51 [Abstract] ( 11 ) HTML (0 KB)  PDF-CHN (1350 KB)  PDF-ENG  (0 KB)  ( 10 ) | Cite this article
 TOBACCO EQUIPMENT
CHEN Jiakun, DING Wenbin, LIU Yinchu, SHENG Xiaowen
Improvement of moisture exhausting pipeline and design of cleaning system for SJ233B flavoring machine
2020 Vol. 26 (6): 52-60 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (2545 KB)  PDF-ENG  (0 KB)  ( 7 ) | Cite this article
CHEN Tianli, WANG Kui, SHEN Wei, GUI Yuan, CHEN Enbo
Design of microwave detection system of glycerol triacetate in filter rods
2020 Vol. 26 (6): 61-65 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1357 KB)  PDF-ENG  (0 KB)  ( 8 ) | Cite this article
 AGRONOMY AND CURING
ZHANG Xiaoquan, CAI Wenlong, KAN Hongying, XUE Bingjie, JIA Zhenyu, WANG Shuai, WU Yunjie
Differences of carbon and nitrogen metabolism and related gene expression during leaf senescence phase for different flue-cured tobacco varieties
2020 Vol. 26 (6): 66-73 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (1695 KB)  PDF-ENG  (0 KB)  ( 7 ) | Cite this article
LI Yimei, WANG Zhaojun, YAN Xiaoxiao, ZHANG Hongying, LI Xuejun, SUN Jiping, HUANG Mingyue, CUI Hong
Leaf surface chemical component improvement by NtCPS2 gene knock out in tobacco line “8306”
2020 Vol. 26 (6): 74-80 [Abstract] ( 1 ) HTML (0 KB)  PDF-CHN (2923 KB)  PDF-ENG  (0 KB)  ( 4 ) | Cite this article
ZHANG Guangyu, HU Zhiming, CHU Depeng, DAI Yongping, YIN Shaojing, YAN Mingxiong, DUAN Yan, SUN Kang, Naila Ilyas, YOU Xiangwei, LI Yiqiang
Regulation of biochar on rhizosphere soil microecology and its control effect on tobacco bacterial wilt
2020 Vol. 26 (6): 81-88 [Abstract] ( 8 ) HTML (0 KB)  PDF-CHN (2341 KB)  PDF-ENG  (0 KB)  ( 4 ) | Cite this article
GU Huizhan, MU Mingxin, SHI Hongtao, HE Jixian, WEI Yongtao, ZHUANG Ye, WANG Xiaodong
Some thoughts on ‘Zhongkeyan’ cultivation of flue-cured tobacco
2020 Vol. 26 (6): 89-96 [Abstract] ( 11 ) HTML (0 KB)  PDF-CHN (1417 KB)  PDF-ENG  (0 KB)  ( 15 ) | Cite this article
 MODERN TOBACCO AGRICULTURE
LI Xiang, LIU Yanxia, SUN Guangjun, LI Guanglei, WANG Junfei, MU Dongsheng, JIANG Chaoying, JIN Xiang, JIAO Jian, XIA Zhilin, GUO Liang, CHEN Fenglei, SHEN Hong
Study on driving forces for tobacco farmers to increase land transfer scale under planned management
2020 Vol. 26 (6): 97-104 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (1509 KB)  PDF-ENG  (0 KB)  ( 6 ) | Cite this article
 ECONOMY AND MANAGEMENT
MENG Bo, HE Min, SHEN Si
Discussion on the application of rail-road multimodal transportation in the field of cigarette transportation
2020 Vol. 26 (6): 105-110 [Abstract] ( 9 ) HTML (0 KB)  PDF-CHN (1279 KB)  PDF-ENG  (0 KB)  ( 5 ) | Cite this article
LIU Wei, LI Ban, LIU Lu
Study on pressure model of epidemic prevention and control for cigarette transportation and distribution of tobacco enterprises under public health events
2020 Vol. 26 (6): 111-117 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (20285 KB)  PDF-ENG  (0 KB)  ( 9 ) | Cite this article
 
2020 Vol. 26 (6): 118-120 [Abstract] ( 5 ) HTML (0 KB)  PDF-CHN (1518 KB)  PDF-ENG  (0 KB)  ( 8 ) | Cite this article
2020  Vol. 26 No. 6    Published: 28 December 2020
TOBACCO AND SMOKE CHEMISTRY    MANUFACTURING TECHNOLOGY    TOBACCO EQUIPMENT    AGRONOMY AND CURING    MODERN TOBACCO AGRICULTURE    ECONOMY AND MANAGEMENT       
 TOBACCO AND SMOKE CHEMISTRY
WU Shuaifan, CUI Xiuming, HUANG Yan
Study on the interaction of nicotine and benzoic acid
2020 Vol. 26 (6): 1-5 [Abstract] ( 14 ) HTML (0 KB)  PDF-CHN (1209 KB)  PDF-ENG  (0 KB)  ( 33 ) | Cite this article
Abstract:[Objective] This paper aims to explore whether nicotine can bind to benzoic acid to form a stable complex.[Methods] The binding manner was confirmed by Job's Plot method, 1H NMR, FT-IR, ITC and molecular modeling calculation.[Results] 1) The binding ratio of nicotine and benzoic acid was confirmed to be 1:1 by Job's Plot method; 2) The binding manner was further confirmed by 1H NMR & FT-IR. 3) Thermodynamic data (ΔH=﹣26.8 KJ/mol; ΔG=﹣33.99 KJ/mol; ΔS=24.12 J/mol·K) and association constant (Ka=9.021×105 M-1) were obtained by ITC. 4) Binding energy (E=﹣18.64 Kcal/mol) was given by molecular modeling calculation.[Conclusion] The test data showed that nicotine and benzoic acid can form a stable complex with a binding ratio of 1:1, which has a higher decomposition temperature.
ZHAO Guoling, YIN Xinqiang, HUANG Jianguo, GONG Shuguo, DAI Yuangang, ZHONG Kejun, PENG Xinhui
Study on the release of nicotine in different forms in e-cigarette aerosol
2020 Vol. 26 (6): 6-10 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (908 KB)  PDF-ENG  (0 KB)  ( 25 ) | Cite this article
Abstract:[Background] The release laws of free nicotine, adsorbed nicotine, inclusion nicotine, and different proportions of nicotine and tartaric acid concoctions in electronic cigarette aerosol were studied.[Methods] The changes of nicotine content and pH value in e-cigarette liquid containing different forms of nicotine were measured. The release of different forms of nicotine in electronic cigarette aerosol as well as the difference in sensory quality evaluation were compared.[Results] (1) Compared with e-cigarette liquid containing free nicotine, the addition of cyclodextrin-modified graphene oxide, carbon oxide nanotubes, carboxymethyl cellulose and hydroxypropyl-ß-cyclodextrin could decrease the concentration of nicotine but had little effect on pH valve of the e-cigarette liquid. However, the addition of tartaric acid would decrease the pH value of the e-cigarette liquid and reduce the concentration of nicotine. (2) The addition of carbon oxide nanotubes to the e-cigarette liquid decreased the release stability of nicotine in the aerosol than e-cigarette liquid containing free nicotine. Addition of cyclodextrin modified graphene oxide and carboxymethyl cellulose and hydroxypropyl-ß-cyclodextrin led to a better release stability of nicotine in the aerosol than e-cigarette liquid containing free nicotine. The addition of tartaric acid to the e-cigarette liquid reduced the amount of nicotine released in the aerosol, and improved the release stability of nicotine in the aerosol than e-cigarette liquid containing free nicotine. (3) In terms of sensory quality evaluation, compared with e-cigarette liquid containing free nicotine, e-cigarette liquid containing hydroxypropyl-ß-cyclodextrin had lower throat impact. The e-cigarette liquid containing tartaric acid had a weaker nicotine breath, and the throat strength and irritation were significantly reduced.[Conclusion] Adding appropriate amounts of hydroxypropyl-ß-cyclodextrin and carboxymethyl cellulose and tartaric acid to e-cigarette liquid containing free nicotine can effectively increase the release stability of nicotine in the e-cigarette aerosol and improve the sensory quality of e-cigarette products.
ZHAO Deqing, DENG Yong, GUO Linqing, SHI Jianyang, WANG Shuaipeng, LIANG Kun, HAN Donglin, HUANG Yuchuan
Rapid determination of glycerol in HTP cigarette with enzyme electrode method
2020 Vol. 26 (6): 11-17 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (1242 KB)  PDF-ENG  (0 KB)  ( 16 ) | Cite this article
Abstract:[Objective] This study aims to realize rapid and accurate determination of glycerol content in HTP cigarette.[Methods] Enzyme electrode method based on biosensors was established, and the detection results from enzyme electrode method were compared with those of GC method. The transfer rate of glycerol during smoking was also measured.[Results] (1) There was a significant linear correlation (R2=0.9998) in standard curves of enzyme electrode method, and the LOD, LOQ, recovery rate and RSD were 0.045mg/mL, 0.149 mg/mL, 97.64%~102.32%, and 2.15% (n=6), respectively, showing improved repeatability. (2) There was no significant difference between the test results from enzyme electrode method and those from GC method, and the accuracy of the established method was better. (3)The transfer rate of glycerol during smoking process was within the range from 33% to 41%.[Conclusion] Compared with GC method, the enzyme electrode method has high specificity, high stability, fast detection and high sensitivity, which can be suitably applied in determining the amount of glycerol in heat-not-burn cigarette and corresponding tobacco material.
LI Bo, ZHANG Shuming, SONG Shihao, WANG Mingxia, LIU Zhen, WANG Hui, ZHANG Congyue, YUE Mingfei, YIN Shaokai
Sensory evaluation of mixed smoke of traditional cigarette and electronic cigarette: Research on transboundary electronic cigarette based on fuzzy mathematics
2020 Vol. 26 (6): 18-24 [Abstract] ( 5 ) HTML (0 KB)  PDF-CHN (1477 KB)  PDF-ENG  (0 KB)  ( 18 ) | Cite this article
Abstract:Taking mixed smoke of traditional cigarette and e-cigarette as research objects, with taste, aroma, harmony, miscellaneous gas, irritation, aftertaste, sweet and greasy feeling and smoke amount as sensory evaluation indexes, and excellent, very good, medium, poor as the four ratings, in this paper, analytic hierarchy process (AHP) was used to determine the weights of sensory evaluation indexes for mixed smoke, and fuzzy mathematics based comprehensive sensory evaluation was carried out for 8 samples. The reproducibility test proves that the method is reliable and stable, and can realize scientific, reasonable and quantitative evaluation the mixed smoke of traditional cigarette and e-cigarette.
LIU Xiucai, LAN Hongqiao, LIN Yan, LI Qiaoling, LIAN Fenyan, ZHENG Quanxing, YE Zhongli, ZHONG Tinggui, SU Mingliang
Distribution and transfer of menthol in mentholated cigarette with breakable capsule in filter
2020 Vol. 26 (6): 25-31 [Abstract] ( 10 ) HTML (0 KB)  PDF-CHN (1182 KB)  PDF-ENG  (0 KB)  ( 14 ) | Cite this article
Abstract:In order to study the distribution and transfer of menthol in mentholated cigarette with breakable capsule in filter, menthol content in filter, breakable capsule, cut tobacco and cigarette paper, the puff-by-puff transfer amount of menthol in mainstream smoke, and the variation of menthol content with time were investigate in three samples.. The results showed that:1) The menthol content in cigarette can be ranked as:cigarette paper << tobacco < filter < breakable capsule, indicating high proportion distribution near cigarette filter is beneficial to menthol migration in mainstream smoke; 2) The menthol transfer rate in mainstream smoke was 8.90%~10.25%, and the interception rate of filter was 67.18%~73.07%, indicating that most menthol was trapped by the filter and effective utilization rate of menthol was low; 3) As cigarettes were smoked puff-by puffy, the transfer amount of menthol increased gradually. The first three puffs increased rapidly, the fourth puff began to increase steadily, and the last or penultimate puff reached the maximum, which is consistent with cigarette smoking process from weak to strong cool feeling; 4) There was no significant change of menthol in the breakable capsule within 12 months of storage. Menthol in filter, cut filler and mainstream smoke did not reduce significantly in the first six months, but decreased after 9 months and further decrease in a faster pace after 12 months, indicating the best shelf-life of mentholated cigarette is one year.
 MANUFACTURING TECHNOLOGY
HE Wei, FAN Jianqiang, MU Wenjun, CHEN Yiqiang, BAO Kexiang, LIN Jian, ZHANG mingqian, ZHANG Ruiqiang, LIU Jingchuan, SONG Jizhen, WANG Chunlu
Construction of green aging technology system for flue-cured tobacco strips using four-stage controlled atmosphere method
2020 Vol. 26 (6): 32-44 [Abstract] ( 5 ) HTML (0 KB)  PDF-CHN (3149 KB)  PDF-ENG  (0 KB)  ( 14 ) | Cite this article
Abstract:[Background] Flue-cured tobacco strips aging plays an important role in improving the tobacco quality and use value of tobacco. Natural aging is the most widely used storage and aging method at present. However, it still suffers from some problems, such as shorter suitable aging duration, easy occurrence of mildew and storage pests and chemical pesticides usage in fumigation.[Methods] Taking the flue-cured tobacco strips from the main tobacco producing areas in Fujian, Yunnan, Argentina as test materials, the optimal oxygen concentrations for controlled-atmosphere (CA) pest and mildew control stage, CA aging stage and CA quality maintenance stage were studied. The sensory quality, appearance quality and pH value were compared between natural aging and Four-Stage CA aging method.[Results] Test data show:1) The tobacco beetles and mildew can be effectively controlled using low oxygen concentration (≤ 2%) for 90 days. After that, the oxygen concentration was adjusted to 7%~9% for more than 120 days, this CA aging stage can significantly improve the sensory quality and delay the over-darkening of tobacco strips. At last, reducing the oxygen concentration below 2% and maintaining it until the tobacco stack was opened; 2) The proposed technology not only improved the tobacco aging quality, but also extended the suitable aging duration by at least 15 months compared to the natural aging. The decrease rate of pH values were also delayed; 3) The relative humidity was relatively stable inside the CA stacks. The oxygen concentration inside the stack can be artificially controlled to reach the expected concentration, while carbon dioxide inside the stack rose steadily.[Conclusion] A four-stage novel CA aging technology is established in this work. This technique not only avoids the use of highly toxic chemical pesticides in fumigation to kill pests, but also improves the aging quality of flue-tobacco strips, extends its suitable and optimal use period, which makes clear the scientific use strategy of flue-tobacco strips in different producing areas, providing a new idea for the storage of flue-tobacco strips.
GAO Zhanyong, WANG Hui, YANG Jingjin, LU Honglin, WANG Yuzhen, HUA Yikun, LI Siyuan, YANG Jiadong, GAO Hui
Chromatic value of different segments of main vein of flue-cured tobacco and analysis by Fisher optimal segmentation method
2020 Vol. 26 (6): 45-51 [Abstract] ( 11 ) HTML (0 KB)  PDF-CHN (1350 KB)  PDF-ENG  (0 KB)  ( 10 ) | Cite this article
Abstract:[Objective] This study aims to analyze the changes in the chromatic values of different segments of main vein of flue-cured tobacco leaves so as to provide references for the differentiated processing and classification of tobacco stems.[Methods] The main veins in Yunyan87 B3F and C3F cured tobacco leaves were equally divided into 6 segments. The chromatic values L* (lightness value), a* (redness value), b* (yellowness value) and E (total color difference value) of the tobacco stem powder of each segment were measured by a colorimeter. Single factor analysis of variance and regression analysis were carried out to study the difference and change trend of the chromatic values of tobacco stems at different segments. Finally, the optimal segmentation ratio of the main veins of the two types of tobacco leaves was analyzed by Fisher optimal segmentation method.[Results] 1)There were significant differences in chromatic values L*, a*, b* and E values of tobacco stems at different segments of main vein for the same tobacco leaf. Through comparison of chromatic values of the same segment in main veins of B3F and C3F tobacco leaves, it was found that the L* value was C3F> B3F, and a*, b*, E values were B3F> C3F. 2) From stem base to stem tip, the best segment ratio of the main vein of B3F tobacco can be divided into 1/6, 3/6, 1/6, 1/6, and the best segment ratio of C3F tobacco can be divided into 4/6, 1/6, 1/6.[Conclusion] There are significant differences and regular trends in the chromatic values of different segments of tobacco main vein. The tobacco stems in the leaf base and the middle part of the leaf are more ideal raw materials for stem cutting.
 TOBACCO EQUIPMENT
CHEN Jiakun, DING Wenbin, LIU Yinchu, SHENG Xiaowen
Improvement of moisture exhausting pipeline and design of cleaning system for SJ233B flavoring machine
2020 Vol. 26 (6): 52-60 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (2545 KB)  PDF-ENG  (0 KB)  ( 7 ) | Cite this article
Abstract:For the moisture exhaustpipeline systemof current SJ233B flavoring machine in the tobacco primary processing workshop, the moisture exhaust fan impeller is easy to catch fire, and the moisture exhaust pipeline is easy to accumulate scale, which is difficult to clean, and the smoke scale and smoke oil in the moisture exhaust pipeline easily fall into the finished tobacco, causing quality defects. To this end, the original moisture exhaust piping system is improved by designing a novel cleaning system. Based on the principle of cyclone separator, the separation of soot particles and fragrance tidal air flow is realized. By using manual control cleaning and PLC program to control the blow, the cleaning control mode of the moisture exhaust pipeline is realized. The results show that the outbreak of a fire did not occur again after the improvement of the moisture exhaust pipeline system. The cleaning time of the moisture discharge pipeline was reduced to 3 min/time. Suppose the annual production days is 300 d, it saved about 424 tons of water and about 5256 degrees of electricity. Moreover, the application of the cleaning system can avoid the repeated disassembly and installation during cleaning the moisture discharge pipeline, eliminate product quality hazards caused by cleaning problems, improve the operation stability of the moisture exhaust system, thus guaranteeing the final product quality.
CHEN Tianli, WANG Kui, SHEN Wei, GUI Yuan, CHEN Enbo
Design of microwave detection system of glycerol triacetate in filter rods
2020 Vol. 26 (6): 61-65 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1357 KB)  PDF-ENG  (0 KB)  ( 8 ) | Cite this article
Abstract:In order to solve the problems in detection of glycerol triacetate content in filter rods, a new microwave resonance amplitude-frequency characteristic analysis technology was used to design a microwave online detection system for glycerol triacetate content in filter rods. The proposed system can detect and calculate the glycerol triacetate content of each filter rod in the production process, accurately eliminate the filter rod with excessive glycerol triacetate content. The application results show that the detection accuracy of the online detection system is 0.01%, the maximum error between the content displayed by the triacetin detection and the actual content is 0.18%, and the defect removal accuracy rate reaches is up to 100%. This avoids unqualified filter rods from flowing into the next production process and provides technical support for the production of filter rods to improve the quality process control level.
 AGRONOMY AND CURING
ZHANG Xiaoquan, CAI Wenlong, KAN Hongying, XUE Bingjie, JIA Zhenyu, WANG Shuai, WU Yunjie
Differences of carbon and nitrogen metabolism and related gene expression during leaf senescence phase for different flue-cured tobacco varieties
2020 Vol. 26 (6): 66-73 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (1695 KB)  PDF-ENG  (0 KB)  ( 7 ) | Cite this article
Abstract:[Background] This study aims to study the difference and relationship of carbon and nitrogen metabolism and related gene expression in the leaf senescence stage of different flue-cured tobacco varieties.[Methods] Taking the middle leaves of four flue-cured tobacco varieties with different yellowing performances in the field as materials, this paper analyzed the changes of the content of plastid pigments and intermediates of carbon and nitrogen metabolism during the process from leaf maturation to senescence, and detected the expression levels of related genes.[Results] The content of chlorophyll and carotenoid in the leaves of Yuyan10 was low, and the decrement reached the maximum with the increase of leaf age. The carotenoid cleavage dioxygenase gene (CCD) expression level was high, the decrement of NC89 was smaller, and the content was higher, and the CCD expression level was lower. The content of starch in leaves reached a peak at 50-60 d of leaf age, and then began to decline. From the peak to the 70 d of leaf age, the decrement in starch content of Yuyan 10 (38.65%) was relatively large, and the expression levels of its starch synthesization-related genes, including granulebound starch synthase gene (GBSS1) and starch branching enzyme gene (SBE), were relatively low. The contents of total sugar and reducing sugar also decreased significantly from the peak to the 70 d of leaf age. The contents of starch, total sugar, reducing sugar and total carbon in NC89 were relatively high. The carbon metabolism indexes of Yunyan87 and K326 were in the middle level between those of Yuyan 10 and those of NC89. The total nitrogen, C/N ratio and nicotine content in Yuyan 10 leaves from leaf maturation to senescence were significantly lower than those of NC89. Consistently, nitrogen transfer related cytoplasmic glutamine synthase gene (GS1-3) had significantly higher expression level in Yuyan10 than in NC89 after 40 days of leaf age, and nitrogen assimilation related chloroplast glutamine synthase gene (GS2), glutamate dehydrogenase gene (GDH1) and nitrate reductase gene (NR) had the relatively low expression levels in Yuyan10.[Conclusions] The senescence characteristics of different flue-cured tobacco varieties are related to carbon and nitrogen metabolism. The varieties with rapid leaf senescence rate have weaker starch synthesis and metabolism, lower starch content, weaker nitrogen assimilation, stronger ability of nitrogen reutilization and retransfer metabolism, and lower carbon and nitrogen metabolism.
LI Yimei, WANG Zhaojun, YAN Xiaoxiao, ZHANG Hongying, LI Xuejun, SUN Jiping, HUANG Mingyue, CUI Hong
Leaf surface chemical component improvement by NtCPS2 gene knock out in tobacco line “8306”
2020 Vol. 26 (6): 74-80 [Abstract] ( 1 ) HTML (0 KB)  PDF-CHN (2923 KB)  PDF-ENG  (0 KB)  ( 4 ) | Cite this article
Abstract:Tobacco line 8306 is an important breeding germplasm for flue-cured tobacco with high aroma content. However, due to the existence of labdanoids in its glandular trichome exudates, the sun-cured tobacco scent is mixed in its smoke, which limits its application. Thus, NtCPS2, the key gene of labdanoids biosynthesis was knocked out using CRISPR/Cas9 technology in this study and a mutant, named 8306-1, was obtained. 8306-1 carries a single nucleotide insertion in the gRNA region, which resultes a premature stop codon and function loss of NtCPS2. Compared with 8306, 8306-1 showed normal growth and development, and no significant changes of trichome type and density were found. GC-MS analysis showed that no Z-abienol and labdene-diol were detected in 8306-1 glandular trichome exudates, while no significant changes were observed for the content of other components such as cembratrien-ol, cembratrien-diol and sucrose ester as compared with the control. RT-PCR analysis showed that there was no significant difference in the expression of MEP pathway genes such as NtDXS, NtDXR, NtCMS, and cembranoids biosynthesis pathway gene NtCYC and NtCYP71D16 between 8306-1 and 8306. Our results showed that knock out of NtCPS2 gene can effectively down-regulate the biosynthesis of labdanoids, but has no obvious influence on other chemical components of leaf surface. Therefore, NtCPS2 is an ideal target gene for improvement of tobacco leaf chemical components and high aroma flue-cured tobacco breeding.
ZHANG Guangyu, HU Zhiming, CHU Depeng, DAI Yongping, YIN Shaojing, YAN Mingxiong, DUAN Yan, SUN Kang, Naila Ilyas, YOU Xiangwei, LI Yiqiang
Regulation of biochar on rhizosphere soil microecology and its control effect on tobacco bacterial wilt
2020 Vol. 26 (6): 81-88 [Abstract] ( 8 ) HTML (0 KB)  PDF-CHN (2341 KB)  PDF-ENG  (0 KB)  ( 4 ) | Cite this article
Abstract:[Objective] This paper aims to explore the regulation of different amounts of biochar on rhizosphere soil microecology and its control effect on tobacco bacterial wilt.[Methods] The dilution plate method was used to study the adsorption effect of biochar on Ralstonia solanacearum and its influence on growth and motility of Ralstonia solanacearum. Pot experiments were carried out to test the impact of biochar on the incidence of bacterial wilt and physical and chemical properties of soil. Flux sequencing technology was used to compare the differences in soil bacterial communities between treatments.[Results] Test data showed that:(1) Biochar had an inhibitory effect on Ralstonia solanacearum, which could adsorb Ralstonia solanacearum and inhibit its growth and motility. The effect was significant when the dosage exceeded 1.5%; (2) Biochar improved soil pH, organic matter, available phosphorus and available potassium content, and promoted the growth of tobacco. Correlation analysis found the improvement of soil pH and chemical properties by biochar was the main reason for inhibiting the occurrence of tobacco bacterial wilt; (3) Biochar enhanced the Alpha diversity of the rhizosphere soil microbial community and the relative abundance of Streptomyces in Actinomycetes; (4) The diversity of rhizosphere soil bacterial structure had a certain correlation with the physical and chemical properties of the soil, in which available potassium had the largest effect.[Conclusion] Through the inhibition of Ralstonia solanacearum and the improvement of soil microecology, biochar creates an environment conducive to tobacco growth, thus reducing the occurrence of tobacco bacterial wilt.
GU Huizhan, MU Mingxin, SHI Hongtao, HE Jixian, WEI Yongtao, ZHUANG Ye, WANG Xiaodong
Some thoughts on ‘Zhongkeyan’ cultivation of flue-cured tobacco
2020 Vol. 26 (6): 89-96 [Abstract] ( 11 ) HTML (0 KB)  PDF-CHN (1417 KB)  PDF-ENG  (0 KB)  ( 15 ) | Cite this article
Abstract:The ideotype of flue-cured tobacco is affected by many factors. Further enriching and perfecting the connotation, cultivation theory and technical system of flue-cured tobacco ‘Zhongkeyan’ is an important way to achieve the coordinated development of multiple goals such as characteristics, high quality, suitable yield, high efficiency, and to promotethe level of raw material security.This article systematically analyzes the current research progresses on the regulation theory and technology of ideotype of flue-cured tobacco, and combines the new situation that ‘Zhongkeyan’ are vigorously cultivatied in various tobacco areas in China, enriches the connotation and index system of ‘Zhongkeyan’ from the perspective of system theory and large-scale agriculture, analyzes the factors that affect the ideal plant type of flue-cured tobacco, and proposes to cultivate differentiation by coordinating the relationship between plant type, environment and measures. ‘Zhongkeyan’ approach, and prospects for the development of tobacco leaf production in the future, with a view to providing theoretical guidance for the cultivation of ideal flue-cured tobacco plant types and the sustainable development of modern tobacco agriculture.
 MODERN TOBACCO AGRICULTURE
LI Xiang, LIU Yanxia, SUN Guangjun, LI Guanglei, WANG Junfei, MU Dongsheng, JIANG Chaoying, JIN Xiang, JIAO Jian, XIA Zhilin, GUO Liang, CHEN Fenglei, SHEN Hong
Study on driving forces for tobacco farmers to increase land transfer scale under planned management
2020 Vol. 26 (6): 97-104 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (1509 KB)  PDF-ENG  (0 KB)  ( 6 ) | Cite this article
Abstract:[Background] With the continuous improvement of large-scale tobacco planting and the further advancement of modern tobacco agriculture, it is important to explore the willingness of tobacco farmers in land transfer and relevant influence factors in Guizhou Province under planned production conditions.[Methods] Based on four aspects of tobacco farmers' own conditions, natural endowment of tobacco fields, current status of land transfer market, and tobacco farmers' security system, logistic multiple linear regression analysis was used to construct a discrete variable model affecting tobacco farmers' willingness to increase land scale.[Results] The study found that the land transfer situations of the two aroma-type tobacco areas are significantly different. The annual land rent, age, and education level of tobacco farmers were extremely significant negative influencing factors for the honey-sweet-type tobacco area, whereas household labor and tobacco income are extremely significant positive influencing factors for the sweet-fragrance tobacco area. The income from tobacco cultivation is a common positive influence factor for the two flavor type tobacco areas, but varies in the degree of influence. In the honey-sweet tobacco area, the increase in annual rent of 10 yuan per mu of tobacco land will reduce the farmers' willingness of increasing planting land scale by 6.65%. In the sweet-fragrance tobacco area, every 1%increase in professional service level will increase the probability of land transfer by 0.39%.[Conclusion] In summary, it is recommended to ensure land transfer norms, maintain relatively stable land rents for a longer period of time, strengthen the training for professional tobacco farmers and improve service levels, build high-standard basic tobacco fields for increasing tobacco farmers' income.
 ECONOMY AND MANAGEMENT
MENG Bo, HE Min, SHEN Si
Discussion on the application of rail-road multimodal transportation in the field of cigarette transportation
2020 Vol. 26 (6): 105-110 [Abstract] ( 9 ) HTML (0 KB)  PDF-CHN (1279 KB)  PDF-ENG  (0 KB)  ( 5 ) | Cite this article
Abstract:To promote green, safe and intensive transportation, the key is to optimize transport structure. This means we should take the advantages of railway transportation and lower the dependence degree of road transportation in long distance transportation. This paper investigates the necessity and feasibility of rail-road multimodal transportation of cigarette by demonstrating the operation mode of railroad multimodal transportation and the characteristics of cigarette transport.
LIU Wei, LI Ban, LIU Lu
Study on pressure model of epidemic prevention and control for cigarette transportation and distribution of tobacco enterprises under public health events
2020 Vol. 26 (6): 111-117 [Abstract] ( 7 ) HTML (0 KB)  PDF-CHN (20285 KB)  PDF-ENG  (0 KB)  ( 9 ) | Cite this article
Abstract:Under public health emergencies, cigarette transportation and distribution has become a key link of epidemic prevention and control due to their wide-range and strong interaction. The cigarette transportation and distribution business of tobacco manufacturing and commercial enterprises covers more than 300 cities and more than 5 million retail clients in China. The pressure of epidemic control in such link is rapidly increasing. Based on risk management theory and RBS method and using the national coronavirus disease 2019 (COVID-19) epidemic report data and tobacco industry economic operation data, A three-dimensional index system for urban epidemic risk classification is built from the three dimensions of probability, severity and sensitivity of tobacco industry in this paper. Then, combined with the data of cigarette transportation and distribution operations, we designed the risk index of the imported epidemic cases, and calculated the pressure index of the epidemic prevention faced by enterprises. Eventually, the pressure model of epidemic prevention and control of the tobacco manufacturing and commercial enterprises under the public health incident is established, which provides a basis for tobacco companies to scientifically assess the pressure of epidemic prevention, reasonably classify risk levels, and effectively formulate hierarchical strategies for epidemic prevention and control.
 
2020 Vol. 26 (6): 118-120 [Abstract] ( 5 ) HTML (0 KB)  PDF-CHN (1518 KB)  PDF-ENG  (0 KB)  ( 8 ) | Cite this article
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