Acta Tabacaria Sinica

2021 Vol. 27 No. 2 Published: 28 April 2021
TOBACCO AND SMOKE CHEMISTRY TOBACCO EQUIPMENT AGRONOMY AND CURING BIOTECHNOLOGY ECONOMY AND MANAGEMENT CIGAR COLUMN

TOBACCO AND SMOKE CHEMISTRY

LIU Yibo, CHEN Zhihong, LIU Xi, HU Jing, LI Feng

Analysis of the rate discharge performance of electronic cigarette batteries and the influence on aerosol release behavior

2021 Vol. 27 (2): 1-7 [Abstract] ( 6 ) HTML (0 KB) PDF-CHN (1190 KB) PDF-ENG (0 KB) ( 20 ) | Cite this article

ZHANG Mengyue, LIU Deshui, WANG Jun, ZHAO Yuanyuan, ZHOU Jun, MA Yanjun, LU Ximei, SHI Hongzhi

Effects of packaging methods on the formation of TSNAs in tobacco during storage

2021 Vol. 27 (2): 8-19 [Abstract] ( 3 ) HTML (0 KB) PDF-CHN (1552 KB) PDF-ENG (0 KB) ( 7 ) | Cite this article

TOBACCO EQUIPMENT

ZHANG Ting, SHU Meng, WANG Zhijun, CHEN Guohui, SHEN Donghui, WANG Xiang

Design of six-side beautifying device of cigarette carton transparent paper with single station

2021 Vol. 27 (2): 20-26 [Abstract] ( 13 ) HTML (0 KB) PDF-CHN (1850 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

AGRONOMY AND CURING

TANG Chungui, ZHAI Zhengguang, ZHONG Yuefeng, LI Jianyong, PENG Mengxiang, LI Juan

Distribution and availability of soil trace elements and their relationship with bulk density in paddy-tobacco growing area of Hunan province

2021 Vol. 27 (2): 27-34 [Abstract] ( 2 ) HTML (0 KB) PDF-CHN (1786 KB) PDF-ENG (0 KB) ( 3 ) | Cite this article

XIAO Qingli, SUN Lanxi, DAI Xianqiang, CHEN Kunyan, YANG Shimeng, WANG Changguo, YANG Chao, DAI Ya, DING Wei

Spatial-temporal distribution characteristics of soil moisture in dryland tobacco fields in the Wuling mountain area

2021 Vol. 27 (2): 35-44 [Abstract] ( 3 ) HTML (0 KB) PDF-CHN (2005 KB) PDF-ENG (0 KB) ( 9 ) | Cite this article

DENG Jianqiang, REN Xiaohong, ZUO Mei, FAN Jun, XIA Pengliang, HUANG Yong, XIANG Bikun

Effects of different external nitrogen forms on acidolysis organic nitrogen fractions in tobacco-planting soil

2021 Vol. 27 (2): 45-52 [Abstract] ( 4 ) HTML (0 KB) PDF-CHN (1309 KB) PDF-ENG (0 KB) ( 3 ) | Cite this article

BIOTECHNOLOGY

YANG Jiahan, ZHANG Binghui, GU Gang, YANG Xuanshong, QIN Mingyue, XIE Xiaofang

Genome-wide analysis of bZIP gene family and their expression in different maturity stages of tobacco

2021 Vol. 27 (2): 53-64 [Abstract] ( 8 ) HTML (0 KB) PDF-CHN (3460 KB) PDF-ENG (0 KB) ( 9 ) | Cite this article

SONG Jiaqian, XU Liang, WANG Yuelin, YE Xiefeng, ZHONG Chuan, LI Jun, ZHONG Zizheng, YAO Pengwei, WANG Jing, LU Jian

Study on the mechanism of exogenous thymol to improve tobacco seedlings' resistance to salinity stress

2021 Vol. 27 (2): 65-71 [Abstract] ( 4 ) HTML (0 KB) PDF-CHN (3218 KB) PDF-ENG (0 KB) ( 3 ) | Cite this article

LI Xiaojie, LIU Chang, LI Chengjun, YANG Lijun, QIU Rui, SONG Ruifang, CHEN Yuguo, BAI Jingke, LI Shujun

Screening and evaluation of specific primers for Ralstonia solanacearum based on RAPD Technology

2021 Vol. 27 (2): 72-78 [Abstract] ( 6 ) HTML (0 KB) PDF-CHN (2268 KB) PDF-ENG (0 KB) ( 2 ) | Cite this article

CHEN Jinfen, GU Kaiyuan, JIA Yuhao, CHENG Xin, ZOU Congming, XIE Xiaoyu

Effects of exogenous betaine and salicylic acid on the physiological characteristics of tobacco under manganese stress

2021 Vol. 27 (2): 79-86 [Abstract] ( 2 ) HTML (0 KB) PDF-CHN (1731 KB) PDF-ENG (0 KB) ( 2 ) | Cite this article

ECONOMY AND MANAGEMENT

LI Bin, HUANG Zhongyu, YIN Hong

Compilation and empirical exploration of cigarette market potential index from the perspective of new economic geography

2021 Vol. 27 (2): 87-92 [Abstract] ( 12 ) HTML (0 KB) PDF-CHN (1384 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

SHI Yuntao, GAO Qi, ZHANG Congqun

Study on relation among value perception, trust propensity and integrity-support group members' continuous intention

2021 Vol. 27 (2): 93-101 [Abstract] ( 6 ) HTML (0 KB) PDF-CHN (1735 KB) PDF-ENG (0 KB) ( 11 ) | Cite this article

CIGAR COLUMN

FAN Ningbo, CAI Bin, ZHANG Ruina, GAO Huajun, ZOU Yuhang, SONG Zhaopeng, LU Xiaochong

The influence of pigment degradation and membrane lipid peroxidation on color change of cigar tobacco during air curing

2021 Vol. 27 (2): 102-108 [Abstract] ( 7 ) HTML (0 KB) PDF-CHN (1373 KB) PDF-ENG (0 KB) ( 29 ) | Cite this article

RONG Shibin, LI Jingjing, ZHAO Yuanyuan, QIN Yanqing, WANG Jun, YANG Xingyou, ZHANG Ruina, TAN Shu, LI Chuansheng, YUAN Yulong, SHI Hongzhi

Effect of artificial fermentation temperature and humidity on chemical composition and aroma quality of cigar filler tobacco

2021 Vol. 27 (2): 109-116 [Abstract] ( 11 ) HTML (0 KB) PDF-CHN (1647 KB) PDF-ENG (0 KB) ( 10 ) | Cite this article

ZHANG Ge, LI Zhihao, DENG Shuaijun, LI Dongliang, ZHANG Liang, CAI Bin, XIANG Xiaohua, WANG Juan, WANG Fan, CHEN Guoqiang, ZHANG Haibo, LIU Haobao

Characterization and succession analysis of bacterial community diversity in different fermentation cycles of Hainan H382 cigar leaf

2021 Vol. 27 (2): 117-126 [Abstract] ( 10 ) HTML (0 KB) PDF-CHN (2450 KB) PDF-ENG (0 KB) ( 7 ) | Cite this article

2021 Vol. 27 No. 2 Published: 28 April 2021
TOBACCO AND SMOKE CHEMISTRY TOBACCO EQUIPMENT AGRONOMY AND CURING BIOTECHNOLOGY ECONOMY AND MANAGEMENT CIGAR COLUMN

TOBACCO AND SMOKE CHEMISTRY

LIU Yibo, CHEN Zhihong, LIU Xi, HU Jing, LI Feng

Analysis of the rate discharge performance of electronic cigarette batteries and the influence on aerosol release behavior

2021 Vol. 27 (2): 1-7 [Abstract] ( 6 ) HTML (0 KB) PDF-CHN (1190 KB) PDF-ENG (0 KB) ( 20 ) | Cite this article

Abstract：[Objective] This study aims to study the current status of battery quality of electronic cigarette and analyze its influence on aerosol release behavior. [Method] In this study, 38 kinds of low-power electronic cigarette were selected research objects, and the rate discharge performance of their batteries was analyzed. [Results] (1) The rate discharge performance of batteries of electronic cigarette was uneven, only 52.0% of collected rechargeable electronic cigarette batteries had a discharge capacity retention rate (DCRR) of more than 80% after 400 cycles, and 38.5% of collected disposable electronic cigarette batteries could not discharge at a normal rate, and abnormalities such as such as batteries leakage and bulge were observed during the test; (2)the 1.2A rate discharge characteristic can reflect the actual working capacity of the battery, and determine the number of puffs of the disposable electronic cigarette. The battery of electronic cigarette has a significant impact on the amount of aerosol release and the puff-by-puff release stability of aerosol in actual work. [Conclusion] It is necessary to pay attention to the quality and safety of electronic cigarette and their core components, and regulate the development of the electronic cigarette industry as soon as possible.

ZHANG Mengyue, LIU Deshui, WANG Jun, ZHAO Yuanyuan, ZHOU Jun, MA Yanjun, LU Ximei, SHI Hongzhi

Effects of packaging methods on the formation of TSNAs in tobacco during storage

2021 Vol. 27 (2): 8-19 [Abstract] ( 3 ) HTML (0 KB) PDF-CHN (1552 KB) PDF-ENG (0 KB) ( 7 ) | Cite this article

Abstract：The storage stage of tobacco leaves and stems is an important period for the formation of tobacco specific nitrosamines (TSNAs). In order to explore the effective methods to inhibit the formation of TSNAs during storage, the effects of different conventional packaging methods and vacuum packaging on TSNAs content in tobacco leaves stored at room temperature and high temperature were studied using burley tobacco, flue-cured tobacco, cigar core leaves and stems as the material. The results showed that the TSNAs content in f burley tobacco leaves after natural storage with different packaging methods was significantly different. The content of TSNAs in tobacco leaves after vacuum packaging was the lowest, which was 45.45% and 46.02% lower than that of carton packaging and plastic packaging, respectively, and the content of neutral aroma components in vacuum-packaged tobacco leaves was higher. After different types of tobacco leaves and stems were stored at high temperature, the content of TSNAs in leaves and stems after conventional packaging increased significantly, but the increase range of TSNAs in different types of tobacco leaves under vacuum packaging was significantly reduced. Compared with tobacco leaves stored at high temperature under conventional packaging, the total TSNAs content in cigar core leaves and stems decreased by 38.52% and 44.80%, respectively that of cigar jacket leaves and stems decreased by 28.57% and 35.86%, respectively, and that of burley tobacco leaves and stems decreased by 40.65% and 20.84%, respectively. The TSNAs content in stems of the above three types of tobacco was significantly higher than that in leaves. The total TSNAs contents in leaves and stems of flue-cured tobacco under vaccum packaging were 21.52% and 38.29% lower than those in leaves and stems under conventional packaging, and the TSNAs content in leaves was significantly higher than that in stems. Vacuum packaging can effectively isolate oxygen and inhibit the formation and accumulation of TSNAs during storage.

TOBACCO EQUIPMENT

ZHANG Ting, SHU Meng, WANG Zhijun, CHEN Guohui, SHEN Donghui, WANG Xiang

Design of six-side beautifying device of cigarette carton transparent paper with single station

2021 Vol. 27 (2): 20-26 [Abstract] ( 13 ) HTML (0 KB) PDF-CHN (1850 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

Abstract：[Objective] Aiming at current problems in cigarette carton transparent paper, such as loose wrapping, damage and blocking, a new type of transparent paper beautifying device was designed in this work. [Methods] The device uses PLC as the main controller and cooperates with the air cylinder. Through the design of elbow bar mechanism, movable iron plate and other components, as well as the optimization of separating device, it can reshape the transparent paper in a one-time and all-round way. [Results] The test shows that the beautifying device can accurately realize the six side beautifying of the transparent paper with single station. The operation of the packaging unit is more smooth, and the defect rate of the strip packaging decreases from 1.71% to 0.90%. [Conclusion] This technology can provide support for improving the production adaptability of packaging unit.

AGRONOMY AND CURING

TANG Chungui, ZHAI Zhengguang, ZHONG Yuefeng, LI Jianyong, PENG Mengxiang, LI Juan

Distribution and availability of soil trace elements and their relationship with bulk density in paddy-tobacco growing area of Hunan province

2021 Vol. 27 (2): 27-34 [Abstract] ( 2 ) HTML (0 KB) PDF-CHN (1786 KB) PDF-ENG (0 KB) ( 3 ) | Cite this article

Abstract：[Objective] This work aims to find out the contents of available Cu, Zn, Fe and Mn in 0 ~ 50 cm soil layer of paddy-tobacco growing area in Hunan Province, and to understand their relationship with bulk density. [Methods] 150 soil profile samples were collected from three paddy-tobacco growing areas in Chenzhou, Hengyang and Changsha. The distribution characteristics, abundance and deficiency status and availability indexes of trace elements in different soil layers were analyzed, and the relationship between soil trace elements and bulk density was clarified. [Results] 1) The contents of available Cu, Zn, Fe and Mn in 0 ~ 10 cm soil layer were the highest, and decreased with the deepening of soil layer; The soil samples with medium and above content in Cu, Zn, Fe and Mn accounted for 98.67%, 56.67%, 69.33% and 56.67%, respectively. 2) The comprehensive availability index of trace elements in the 0 ~ 50cm soil layer from top to bottom showed a layer-by-layer decline law, and the decline range of 20 ~ 30 cm soil layer was the largest. 3) The contents of Cu, Zn and Mn in the 20 ~ 50cm soil layer were significantly negatively correlated with bulk density, and the correlation increased with the deepening of the soil layer. [Conclusion] Soil bulk density is one of the factors limiting the improvement of the availability of trace elements in non surface soil in paddy-tobacco growing area of Hunan province.

XIAO Qingli, SUN Lanxi, DAI Xianqiang, CHEN Kunyan, YANG Shimeng, WANG Changguo, YANG Chao, DAI Ya, DING Wei

Spatial-temporal distribution characteristics of soil moisture in dryland tobacco fields in the Wuling mountain area

2021 Vol. 27 (2): 35-44 [Abstract] ( 3 ) HTML (0 KB) PDF-CHN (2005 KB) PDF-ENG (0 KB) ( 9 ) | Cite this article

Abstract：[Objective] The spatial-temporal distribution characteristics of soil moisture in dry land tobacco fields in the Wuling mountain area are studied, to provide theoretical support for the study of drought resistant cultivation technology in tobacco field in the Wuling mountain area. [Methods] From May to September 2018, contour map, temporal stability analysis and correlation analysis were used to study the spatial-temporal distribution characteristics of soil moisture in dry land tobacco fields in the Wuling mountain area. [Results] 1)The content of soil water on the surface of ridge was the lowest, and the fragmentation of contour map was the weakest. However, with the increase of soil depth, the soil moisture content and the fragmentation contour map increased significantly. The water content of each layer of the furrow soil was higher, and the contour map was also a patch with higher degree of fragmentation. 2) Both ridge and furrow soils were adversely affected by the summer drought, which led to the lowest soil moisture at the end of July. 3) Based on the minimum value of the time stability index, the most time-stable location (MTSL) was obtained, and a regression model between the soil moisture content of the MTSL and the average moisture content of the corresponding soil layer. 4) The correlation analysis showed that there was obvious water exchange between ridge soil and furrow soil, and the exchange capacity increased with the increase of the depth of the furrow soil. [Conclusion] Ridge cultivation and plastic mulching significantly changed the spatial-temporal distribution of soil moisture in dryland tobacco fields, and aggravated the soil water deficit in summer drought. The research results can provide theoretical support for the prediction of soil moisture and the drought resistant cultivation in tobacco field.

DENG Jianqiang, REN Xiaohong, ZUO Mei, FAN Jun, XIA Pengliang, HUANG Yong, XIANG Bikun

Effects of different external nitrogen forms on acidolysis organic nitrogen fractions in tobacco-planting soil

2021 Vol. 27 (2): 45-52 [Abstract] ( 4 ) HTML (0 KB) PDF-CHN (1309 KB) PDF-ENG (0 KB) ( 3 ) | Cite this article

Abstract：[Objective] In this study, the effects of different external nitrogen forms on acidolyzable organic nitrogen fractions (AON) in tobacco-planting soil were evaluated, which provides a theoretical basis for soil conservation and targeted fertilization in tobacco-growing region. [Methods] A 15N tracing incubation experiment of Enshi tobacco-growing soil was carried out to analyze the N transformation of external nitrogen forms (i.e.，ammonia N, nitrate N and amino acid N) in AON as well as it's fractions, including ammonium nitrogen (AN), amino acid nitrogen (AAN), amino sugar nitrogen (ASN) and unknown acidolyzable nitrogen (UAN). [Results] (1) At 0-10 d, 50-70 % ammonia 15N was transformed into AON, while the conversion rate (R) of amino acid 15N was slightly lower (45-55 %), and that of nitrate 15N was only 27-31%. However, there was no difference in R values among the treatments, which were about 30%. (2) The weighted averages 15N of ammonia, nitrate and amino acid in AON were 9.84 mg/kg, 8.40 mg/kg and 10.49 mg/kg, respectively, indicating insignificant difference . However, the weighted average 14N in AON was significant different among treatments, the one of ammonia N was -116.71 mg/kg, while the one of the nitrate N and amino acid N were 28.05 mg/kg and 69.84 mg/kg, respectively. (3) Based on the path analysis of structural equation model, it was found that the AN-ASN and ASN-UAN pathways of ammonia N were enhanced, and r values were both positive, indicating that ASN accelerated the transformation from AN into UAN. However, the r values of AN-AAN、 ANNUAN of amino acid N were negative, and ANN inhibited the transformation from AN to UAN. (4) At 1 d, AON of ammonia N was slightly higher than that of other treatments, but then it decreased rapidly and became significantly lower than that of nitrate N and amino acid N at 30 d. [Conclusion] There was no difference in the transformation of 15N of different external nitrogen forms, but it had a greater impact on the transformation of 14N. Amino acid N and nitrate N could increase the AON by increasing the 14N input from other sources, while ammonia N was not conducive to the accumulation of AON by increasing the 14N output.

BIOTECHNOLOGY

YANG Jiahan, ZHANG Binghui, GU Gang, YANG Xuanshong, QIN Mingyue, XIE Xiaofang

Genome-wide analysis of bZIP gene family and their expression in different maturity stages of tobacco

2021 Vol. 27 (2): 53-64 [Abstract] ( 8 ) HTML (0 KB) PDF-CHN (3460 KB) PDF-ENG (0 KB) ( 9 ) | Cite this article

Abstract：Basic region/leucine zipper motif (bZIP) transcription factors regulate processes including growth, development, senescence and various stress responses in plants. As a model species, the identification of bZIP family in tobacco is meaningful. In this study, a total of 126 NtbZIP genes were identified from the tobacco genome using bioinformatics method. The bZIP family was divided into 11 subgroups with variable number of exons (1-13)，and the members within each subfamily were found to contain similar exon organizations according to the gene structures analysis. The NtbZIP gene contains a highly conserved basic region and a leucine zipper. The bZIPs were classified into 11 subgroups and each subfamily contained the members of bZIP from the both species based on phylogenetic analysis between tobacco and Arabidopsis, and S group was the largest subfamily which contained 43 members of bZIP from the both species. The members of NtbZIPs showed differential expression in tobacco leaves at different maturity stages, and among them, the expression levels of NtbZIP84, NtbZIP115, NtbZIP119 and NtbZIP120 genes decreased precisely corresponding to the increasing of the maturity degrees. Our results provide valuable information for a better understanding of bZIP genes in tobacco and lay a better foundation for further functional exploration of the tobacco bZIP gene family.

SONG Jiaqian, XU Liang, WANG Yuelin, YE Xiefeng, ZHONG Chuan, LI Jun, ZHONG Zizheng, YAO Pengwei, WANG Jing, LU Jian

Study on the mechanism of exogenous thymol to improve tobacco seedlings' resistance to salinity stress

2021 Vol. 27 (2): 65-71 [Abstract] ( 4 ) HTML (0 KB) PDF-CHN (3218 KB) PDF-ENG (0 KB) ( 3 ) | Cite this article

Abstract：[Objective] High salt content in soil seriously affects plant growth and causes salinity stress. In order to explore the exogenous substances that can improve the salt tolerance of tobacco, the research group screened a large number of compounds in the early stage. Thymol with the best alleviation effect was adopted to study the mechanism of salinity stress. [Methods] Using flue-cured tobacco K326 as material, the cell death of root tip and the flow rate of sodium and potassium ion were determined were measured by chemical staining and non-invasive micromeasurement. Based on physiological indexes such as root length, active oxygen content and antioxidant enzyme content of tobacco seedlings, the mechanism of thymol in alleviating salinity stress in tobacco seedlings was explored. [Results] Test data show: 1) The addition of thymol significantly declined the inhibition effect of salinity stress on the root growth of tobacco seedlings; 2) Thymol reduced the accumulation of H2O2 and MDA in plants under salinity stress and alleviated the death of root tip cells caused by salt stress. 3) Thymol alleviated Na+ uptake and K+ efflux caused by salt stress. [Conclusion] Exogenous thymol can alleviate the salt stress-induced damage to tobacco seedlings by reducing the H2O2–induced oxidative damage and maintaining the balance of intracellular sodium and potassium ions.

LI Xiaojie, LIU Chang, LI Chengjun, YANG Lijun, QIU Rui, SONG Ruifang, CHEN Yuguo, BAI Jingke, LI Shujun

Screening and evaluation of specific primers for Ralstonia solanacearum based on RAPD Technology

2021 Vol. 27 (2): 72-78 [Abstract] ( 6 ) HTML (0 KB) PDF-CHN (2268 KB) PDF-ENG (0 KB) ( 2 ) | Cite this article

Abstract：In order to realize rapid diagnosis of Ralstonia solanacearum in the tobacco plant and tobacco field soil, six pairs of specific primers for PCR detection of tobacco bacterial wilt were obtained by RAPD method in this study. According to the tests by six kinds of bacteria (R. solanacearum, Bacillus polymyxa, Bacillus subtilis, Arthrobacter, Pseudomonas fluorescens, Pseudomonas tabaci) and two tobacco varieties (Zhongyan 100 and Yunyan 87), the 6 pairs of specific primers showed good sensitivity, specificity and reliability. The detection sensitivity of DNA in bacterial liquor was 0.42 pg/uL, and the minimum detection rate of soil bacterial wilt was 6×102 cfu/g. The results of PCR amplification using specific primers A1T and S13T showed that the application of two primers realized rapid detection of Ralstonia solanacearum in tobacco planting soil and infected tobacco plants, so that the detetion time was shortened significantly. The results of this study are of great significance for the forecasting and preventing of tobacco bacterial wilt.

CHEN Jinfen, GU Kaiyuan, JIA Yuhao, CHENG Xin, ZOU Congming, XIE Xiaoyu

Effects of exogenous betaine and salicylic acid on the physiological characteristics of tobacco under manganese stress

2021 Vol. 27 (2): 79-86 [Abstract] ( 2 ) HTML (0 KB) PDF-CHN (1731 KB) PDF-ENG (0 KB) ( 2 ) | Cite this article

Abstract：[Objective] This study aims to study the effects of exogenous substances on the physiological characteristics of flue-cured tobacco under manganese stress and determine the optimal substance alleviating manganese stress. [Methods] Pot experiment of fluecured tobacco was carried out under 500 mg/kg manganese sulfate stress. Different concentrations of betaine and salicylic acid were used to alleviate the stress. The manganese content, relative electrical conductivity, malondialdehyde content, antioxidant enzyme activity, osmotic regulation substance content and reactive oxygen production rate of flue-cured tobacco leaves under manganese stress and exogenous substances were analyzed. [Results] The results showed that all the indexes increased significantly under manganese stress, and decreased significantly after the application of betaine and salicylic acid. When the concentration of betaine was 0.20 mol/L and the concentration of salicylic acid was 150 mg/L, the manganese content, relative conductivity, MDA content and catalase activity were the lowest, which was significantly different from the levels under manganese stress, and the osmotic regulator reached the lowest level when the concentration of betaine was 0.15 mol/L. [Conclusion] The comprehensive analysis showed that the alleviating effect of manganese stress was better when the concentration of exogenous betaine was 0.20 mol/L and the concentration of exogenous salicylic acid was 150mg/L.

ECONOMY AND MANAGEMENT

LI Bin, HUANG Zhongyu, YIN Hong

Compilation and empirical exploration of cigarette market potential index from the perspective of new economic geography

2021 Vol. 27 (2): 87-92 [Abstract] ( 12 ) HTML (0 KB) PDF-CHN (1384 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

Abstract：The geographic cigarette market potential index (MP index) has been constructed based on Market Potential Theory of New Economic Geography (NEG) restructured with the average propensity to consume (APC) of tobacco, which interprets the mechanism of socio-economic system in supporting the economical operation of cigarettes market. According to Granger's test, statistical causality between the fluctuation of MP index and the counterpart of cigarette sales has been verified; and the fluctuation of MP index predicts 63.18 percent of cigarette sales fluctuation by panel data regression model. Moreover, a precise prediction of cigarette sale has been obtained by the MP index combined with previous sales. In addition, Market Access Index (MAI) deduced from Core-Periphery model in the frame of NEG theory has been applied to reflect impacts of agglomeration economies on cigarette market potential, which refines the theoretical model of the Market Potential. It can be concluded that the MP index is a sound measure of regional cigarette market potential, which is helpful for high-quality development policy-making.

SHI Yuntao, GAO Qi, ZHANG Congqun

Study on relation among value perception, trust propensity and integrity-support group members' continuous intention

2021 Vol. 27 (2): 93-101 [Abstract] ( 6 ) HTML (0 KB) PDF-CHN (1735 KB) PDF-ENG (0 KB) ( 11 ) | Cite this article

Abstract：On the basis of the theory of reasoned action, this paper explores the influencing factors of members' continuous intention in participating in integrity-support groups and establishes relevant theoretical model. Empirical research was conducted on the basis of the actual construction of integrity-support groups in Suzhou area. The empirical results show that: members' value perception on integritysupport groups will directly and positively affect their trust propensity and continuous intention; higher trust propensity towards integritysupport groups will also positively affect their continuous intention. Although value perception has a direct positive effect on trust propensity, it has no significant effect on member’s continuous intention. Considering that the integrity-support groups is in the early stage of construction and the particularity of the relationship among retail customers, tobacco companies and retail customers, team members' trust propensity towards tobacco companies and other retail customers and their perception of cost for participating in the integrity-support groups are not the influencing factors of their continuous intention. Meanwhile, it is found that the mediating effect of trust propensity is not significant after analyzing the influence effect of latent variable standardization.

CIGAR COLUMN

FAN Ningbo, CAI Bin, ZHANG Ruina, GAO Huajun, ZOU Yuhang, SONG Zhaopeng, LU Xiaochong

The influence of pigment degradation and membrane lipid peroxidation on color change of cigar tobacco during air curing

2021 Vol. 27 (2): 102-108 [Abstract] ( 7 ) HTML (0 KB) PDF-CHN (1373 KB) PDF-ENG (0 KB) ( 29 ) | Cite this article

Abstract：[Objective] This study aims to investigate the effect of pigment degradation and membrane lipid oxidation on the color formation of cigar tobacco during the drying process. [Methods] in this paper, the changes of pigment and membrane lipids in the middle cigar tobacco of "Dexue 7" during air curing were measured, and the relationship between pigment, membrane lipids and the changes of tobacco color was explored. [Results] The results showed that the contents of chlorophyll and carotenoids were gradually degraded with the change of leaf color during the curing process of cigar tobacco leaves, and the proportion of carotenoids in chlorophyll increased at first and then decreased.The membrane lipid content of cigar tobacco leaves (MGDG, DGDG, PL) also decreased, and the DGDG/MGDG gradually increased with the extension of air curing time. There is a significant negative correlation between the color change of cigar tobacco leaves and the content of pigment and membrane lipid. [Conclusion] During the process of air curing, the metabolism of pigment and membrane lipid are two important factors affecting the formation of leaf color.

RONG Shibin, LI Jingjing, ZHAO Yuanyuan, QIN Yanqing, WANG Jun, YANG Xingyou, ZHANG Ruina, TAN Shu, LI Chuansheng, YUAN Yulong, SHI Hongzhi

Effect of artificial fermentation temperature and humidity on chemical composition and aroma quality of cigar filler tobacco

2021 Vol. 27 (2): 109-116 [Abstract] ( 11 ) HTML (0 KB) PDF-CHN (1647 KB) PDF-ENG (0 KB) ( 10 ) | Cite this article

Abstract：In order to explore the influence of fermentation conditions on the aroma quality of cigar filler tobacco, the traditional cigar filler tobacoo variety Shiyan No. 1 was used for artificial fermentation, and temperature and humidity interaction tests were carried out to determine the conventional chemical composition, neutral aroma composition and sensory quality of the samples before and after fermentation. The results showed that: (1) The content of total sugar, reducing sugar, nicotine and total nitrogen in the tobacco leaves after fermentation decreased significantly, and the decreasing amplitude increased with the increase of temperature. (2) The contents of various aroma substances in cigar filler tobacco increased firstly and then decreased with the increase of fermentation temperature, and increased significantly with the increase of humidity. The maximum content reached of 676.75 μg/g was obtained under the condition of 45℃-RH90%, which was increased by 45.36% compared with that before fermentation. (3) Temperature contributed more to the content change of chemical compositions than humidity, especially total sugar and total nitrogen, with a contribution rate of more than 90%, while humidity contributed more to changes in aroma component content and sensory quality. (4) When fermented under the conditions of 45℃~48℃, RH90%, and tobacco leaf moisture content of 30.32%±2.16%, the chemical composition of cigar filler tobacco was more coordinated, the aroma components were excellent, the smoking quality was the best, and the tobacco quality was improved.

ZHANG Ge, LI Zhihao, DENG Shuaijun, LI Dongliang, ZHANG Liang, CAI Bin, XIANG Xiaohua, WANG Juan, WANG Fan, CHEN Guoqiang, ZHANG Haibo, LIU Haobao

Characterization and succession analysis of bacterial community diversity in different fermentation cycles of Hainan H382 cigar leaf

2021 Vol. 27 (2): 117-126 [Abstract] ( 10 ) HTML (0 KB) PDF-CHN (2450 KB) PDF-ENG (0 KB) ( 7 ) | Cite this article

Abstract：[Objective] The purpose of research is to reveal the diversity and succession of bacterial community and to predict the role of bacteria in the process of cigar leaves fermentation. [Methods] In this research, 16S rRNA sequencing technology was used to reveal the community diversity and succession of H382 cigar leaves during fermentation. In addition, PICRUSt was used to predict bacterial community functions. [Results] During the fermentation of cigar leaves, the bacterial community diversity was abundant, including 299 OTUs in seven samples. There was a difference in number of OTU among the different samples, and the highest was 222 in YB_3(sample-3). (2) There was a succession of bacterial community during the fermentation of cigar leaves from the Staphylococcus (YB_2) to oceanobacterillus in YB_3, then to Pseudomonas in YB_4(sample-4), finally to Staphylococcus (YB_5, TB_6, YB_7). (3) The function types of the of PICRUSt results show a little difference in different fermentation periods, but the difference of functional abundance among different samples was significant. [Conclusion] In the fermentation process of H382 cigar leaves, the bacterial community was rich in diversity, and the dominant bacterial community was successional under the experimental conditions. The primary bacterial community succession was from Staphylococcus, Enterococcus, Pantoea to Oceanobacillus Paracoccus, Staphylococcus, Bacillus, followed by Pseudomonas, Enterobacter, and finally to Staphylococcus, Terribacillus. The functional prediction included amino acid transport and metabolism, carbohydrate transport and metabolism. This study is expected to provide reference for the screening of functional bacteria in the microbial fermentation of cigar leaves, and provide theoretical basis for revealing the microbial fermentation mechanism of cigar leaves.