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Acta Tabacaria Sinica
ZHONGGUO YANCAO XUEBAO
Bimonthly
Inaugurated in 1992

Superintended by:
China Association for Science and Technology
Sponsored by:
Chinese Tobacco Society
Editor-in-Chief: YUAN Xingsi
Deputy Editor-in-Chief:
Editors: HA Junli, LIU Xinhua,
             AI Jitao, ZHANG Zheng
English Proofread by:
ZHAO Baidong, XIAO Boxue
Published by:
Editorial Office of Acta Tabacaria Sinica
Address: No.55 Yuetan South Street, Xicheng District, Beijing
Post Code: 100045
Tel: (86-10)63605768, 63605021, 63606237, 63605769, 63606007
Fax: (86-10)63605760
Email: xh-bj@tobacco.gov.cn
ISSN 1004-5708
CN 11-2985/TS
  Editor’s Pick More>>   
Current Issue Full Abstracts Archive
2020  Vol. 26 No. 3    Published: 28 June 2020
HEATED TOBACCO PRODUCTS COLUMN    MANUFACTURING TECHNOLOGY    AGRONOMY AND CURING    PLANT PROTECTION    BIOTECHNOLOGY    ECONOMY AND MANAGEMENT   
 HEATED TOBACCO PRODUCTS COLUMN
YANG Jing, ZHAO Wentao, AI Minghuan, LU Chengwei, Feng Qi, WANG Xu
Performance comparison of different types of cut tobacco materials for heated tobacco products
2020 Vol. 26 (3): 1-8 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (2339 KB)  PDF-ENG  (0 KB)  ( 4 ) | Cite this article
LIU Hong, TAO Liqi, LU Yifeng, WU Da, ZHENG Saijing, CHEN Yong, YAO Heming
Composition analysis of heated tobacco products (HTPs) aerosol by MDGC/MS
2020 Vol. 26 (3): 9-14 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (1714 KB)  PDF-ENG  (0 KB)  ( 5 ) | Cite this article
DENG Jiamin, ZOU Jinhui, ZHENG Xudong, LI Zhiqiang, TANG Jianguo
Battery management system (BMS) for heated tobacco products (HTPs): a review
2020 Vol. 26 (3): 15-23 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1810 KB)  PDF-ENG  (0 KB)  ( 2 ) | Cite this article
GUO Xinyue, YANG Zhanping, SONG Xiaomei, XU Yang
Research progress of cooling technology of heat-not-burn cigarette smoke
2020 Vol. 26 (3): 24-32 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1520 KB)  PDF-ENG  (0 KB)  ( 3 ) | Cite this article
 MANUFACTURING TECHNOLOGY
LIU Xingyu, LIU Deshui, MA Li, MA Yanjun, YANG Zhendong, ZHANG Jie, LIN Lin, BAI Ruoshi, ZHOU Jun
Effect of sodium carbonate buffer treatment on TSNAs accumulation and quality of tobacco leaves in the process of threshing and redrying
2020 Vol. 26 (3): 33-42 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (2548 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
ZHANG Xinyu, CAO Fujun, OU Yafei, LIU Deshui, BAI Ruoshi, YAN Lihong, MA Yanjun, LIN Hui, ZHOU Jun
Comparison of quality of expanded cut burley tobacco with and without baking treatment
2020 Vol. 26 (3): 43-48 [Abstract] ( 5 ) HTML (0 KB)  PDF-CHN (1094 KB)  PDF-ENG  (0 KB)  ( 0 ) | Cite this article
LU Yuhao, WANG Binbin, NIU Ruping, WAN Yunfei, ZHANG Juan, YANG Lei, YANG Ying
Effect of compaction position bias on cigarette processing quality
2020 Vol. 26 (3): 49-53,58 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1160 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
LING Xiaocun, JI Xiong, GONG Xiaojun, ZHOU Feng, HE Zhenkun, FANG Ren, LI Yuanzhang
Improvement of tipping quality of hard-shaped filter cigarettes
2020 Vol. 26 (3): 54-58 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (1422 KB)  PDF-ENG  (0 KB)  ( 0 ) | Cite this article
 AGRONOMY AND CURING
LIU Yong, HUANG Changjun, ZENG Jianmin, XIAO Binggang, YU Haiqin, LI Yongping
Breeding and characteristic of PVY resistant new variety Yunyan301 obtained by directional modification on basis of of Yunyan87 as femal parent
2020 Vol. 26 (3): 59-65 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (1337 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
XIAN Liguo, HUANG Yilan, WANG Songfeng, ZHANG Ying, ZHANG Ye, LIN Zhihui, LIU Hui, XU Xixiang, LIN Shuiliang, SUN Shuaishuai, SUN Fushan
Study on quality and curing characteristics of CB-1 fresh leaves
2020 Vol. 26 (3): 66-73 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (1454 KB)  PDF-ENG  (0 KB)  ( 5 ) | Cite this article
MA Jing, LI Xueli, WANG Peng, SUN Shuguang, MA Junhong, PAN Haodong, YAO Pengwei, WANG Jing, YE Xiefeng
Effects of soil salinity on dry matter weight and absorption and distribution of mineral elements in flue-cured tobacco organs
2020 Vol. 26 (3): 74-83 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1744 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
 PLANT PROTECTION
CHANG Dong, GU Jianguo, JIA Fangfang, MA Wenhui, XU Yueqi, YAN Haitao
Screening and control efficacy of different plant-derived biocontrol bacteria against tobacco black shank disease
2020 Vol. 26 (3): 84-90 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (1680 KB)  PDF-ENG  (0 KB)  ( 3 ) | Cite this article
LONG Ying, LIU Jianping, WU Shaolong, LI Xiaohong
Effects of different tobacco varieties on growth and food utilization efficiency of Spodoptera litura larvae
2020 Vol. 26 (3): 91-97 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (1503 KB)  PDF-ENG  (0 KB)  ( 0 ) | Cite this article
 BIOTECHNOLOGY
HE Conglian, ZHENG Zhiyun, CHEN Yi, ZHAO Gaokun, HE Chenggang, HU Binbin, JIANG Yonglei, ZOU Congming
Screening differentially expressed proteins in response to senescence of tobacco leaves based on iTRAQ
2020 Vol. 26 (3): 98-107 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (3030 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
DING Xiangyu, ZHAO Man, WANG Pengwei, DING Xinhua, CHU Zhaohui, ZHAO Xiangyu, LI Yang
Effect of over-expression of NtHQT on synthesis of caffeoyl quinic acids and flavonols in tobacco
2020 Vol. 26 (3): 108-113 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (2285 KB)  PDF-ENG  (0 KB)  ( 2 ) | Cite this article
 ECONOMY AND MANAGEMENT
CHEN Changhua, HUANG He, YIN Jiankang, LIANG Yan, LI Maobo, HU Yin
Study on logistics benchmark classification system of tobacco business enterprises: A case study of Sichuan province
2020 Vol. 26 (3): 114-120 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (1720 KB)  PDF-ENG  (0 KB)  ( 3 ) | Cite this article
LAI Baoqing, LIU Su, CHEN Huasheng
Design and implementation of standardized post authorization
2020 Vol. 26 (3): 121-126 [Abstract] ( 4 ) HTML (0 KB)  PDF-CHN (1469 KB)  PDF-ENG  (0 KB)  ( 3 ) | Cite this article
2020  Vol. 26 No. 3    Published: 28 June 2020
HEATED TOBACCO PRODUCTS COLUMN    MANUFACTURING TECHNOLOGY    AGRONOMY AND CURING    PLANT PROTECTION    BIOTECHNOLOGY    ECONOMY AND MANAGEMENT   
 HEATED TOBACCO PRODUCTS COLUMN
YANG Jing, ZHAO Wentao, AI Minghuan, LU Chengwei, Feng Qi, WANG Xu
Performance comparison of different types of cut tobacco materials for heated tobacco products
2020 Vol. 26 (3): 1-8 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (2339 KB)  PDF-ENG  (0 KB)  ( 4 ) | Cite this article
Abstract：To develop the raw materials suitable for heated tobacco products, the difference in physicochemical properties of different raw materials should be mastered. In this paper, the optical microscopy and multiple analytical approaches, including differential scanning calorimetry (DSC), thermogravimetry-infrared spectroscopy (TG-IR) and pyrolysis-gas chromatography-mass spectrometry (Py/GC-MS), were used to investigate the thermal properties of cut tobacco and reconstituted tobacco materials structure of heated tobacco products. The results are as follows:①The optical microscope images showed that the surface of cut tobacco of heated tobacco products was wet, the rolling process reconstituted tobacco samples and slurry process reconstituted tobacco samples had relatively tight microstructures, while the dry paper-making reconstituted tobacco samples had porous and loose structure, and the wet paper-making process reconstituted tobacco had obvious fibre structure; ②Thermal performance analysis showed that the heat capacity of tobacco cut, rolling/slurry process reconstituted tobacco samples within temperature range of 50-400℃ was (1023±150)J/g, (1280±200) J/g and (1190±180) J/g, respectively. The lower heat capacity of rolling/slurry process reconstituted tobacco samples comparing with other reconstituted tobacco samples indicated that these two type of reconstituted tobacco samples required less external energy to reach the target temperature. ③TG-IR and Py/GC-MS results showed that different heated tobacco product materials released the similar main components including glycerol, CO2 and nicotine et al. The slurry process reconstituted tobacco sample had more releasing stability between 200℃ and 400℃ compared with other samples. It can be concluded that tobacco cut sample, rolling process reconstituted tobacco sample and slurry process reconstituted tobacco sample had more dense structures in comparison with dry and wet paper-making process reconstituted tobacco samples, which reflected that less adsorbed heat was required to increase the same temperature per unit mass, so it is helpful to increase the filling amount of tobacco material in the process of manufacturing heated tobacco products and save the supply of energy by the heating device.
LIU Hong, TAO Liqi, LU Yifeng, WU Da, ZHENG Saijing, CHEN Yong, YAO Heming
Composition analysis of heated tobacco products (HTPs) aerosol by MDGC/MS
2020 Vol. 26 (3): 9-14 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (1714 KB)  PDF-ENG  (0 KB)  ( 5 ) | Cite this article
Abstract：In order to investigate the chemical composition of heated tobacco products (HTPs) aerosol, a total of 90 chemical compositions such as phenols, organic nitrogen compounds, oxygenated pyrolysis products, acids and alkenes from 17 typical HTPs were analyzed by heart-cutting two-dimensional gas chromatography-mass spectrometry. Results showed that:(1) The main component of HTPs aerosol is close to that of ordinary cigarette smoke, but the release amount is significantly lower, and the release amount per puff of 66.7% of the component types in HTPs aerosols less than 10% of the ordinary cigarette release amount; (2) The components with higher proportions of HTPs aerosol are glycerin (35.8%), nicotine (25.7%), 1,2-propanediol (10.8%), acetic acid (3.1%), furfural (3.1%); the release amount of benzyl alcohol, phenethyl alcohol, solanone, and neophytadiene is close to that of cigarette smoke; (3) The main components in Different types of HTPs aerosols are basically the same, but the release amounts of oxygenated pyrolysis products and phenolic materials are quite different.
DENG Jiamin, ZOU Jinhui, ZHENG Xudong, LI Zhiqiang, TANG Jianguo
Battery management system (BMS) for heated tobacco products (HTPs): a review
2020 Vol. 26 (3): 15-23 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1810 KB)  PDF-ENG  (0 KB)  ( 2 ) | Cite this article
Abstract：As the most promising product in the field of new tobacco products, heated tobacco products (HTPs) represented by IQOS are developing rapidly. In order to further promote the research and development of HTPs products of domestic enterprises, this paper analyzes the design schemes and functional parameters of the existing tobacco battery management systems (BMS) of some tobacco companies at home and abroad. Moreover, this paper reviews the general framework of BMS, battery temperature estimation methods, SOC (State of charge) estimation methods, SOH (State of health) estimation methods, SOF (State of function) estimation methods, SOE (State of energy) estimation methods, IBS (Intelligent battery sensor) parameter detection and information storage, theoretical research and technical points in fault diagnosis and alarm, summarizes the existing BMS research difficulties, and put forward development suggestions based on problems in theoretical research.
GUO Xinyue, YANG Zhanping, SONG Xiaomei, XU Yang
Research progress of cooling technology of heat-not-burn cigarette smoke
2020 Vol. 26 (3): 24-32 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1520 KB)  PDF-ENG  (0 KB)  ( 3 ) | Cite this article
Abstract：Heat-not-burn cigarette is favored by market for release of less harmful components, no smoldering compared with traditional cigarette while maintaining similar smoking quality and smoking habits. Aiming at the technical problem that the inlet flue gas temperature is too high, this paper reviews the related patents on heat-not-burn cigarettes smoke cooling technology in recent years, and summarizes the application problems of cooling materials and structures. On this basis, development direction of heat-not-burn cigarettes cooling technology is prospected.
 MANUFACTURING TECHNOLOGY
LIU Xingyu, LIU Deshui, MA Li, MA Yanjun, YANG Zhendong, ZHANG Jie, LIN Lin, BAI Ruoshi, ZHOU Jun
Effect of sodium carbonate buffer treatment on TSNAs accumulation and quality of tobacco leaves in the process of threshing and redrying
2020 Vol. 26 (3): 33-42 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (2548 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
Abstract：[Background and Objective] Tobacco leaves storage is an important stage in tobacco processing, during which Tobacco Specific N-nitrosamines (TSNAs) in tobacco leaves gradually increase. The purpose of this study is to investigate the influence of sodium carbonate on the accumulation of TSNAs and the quality of tobacco leaves during long-term storage.[Methods] Sodium carbonate buffer was used to adjust the pH value of Maryland and Burley tobacco leaves during threshing and redrying process. After the treated tobacco leaves were naturally stored in 18 months, the tobacco samples were periodically collected to measure the pH value, the content of TSNAs, the appearance of tobacco leaves, the moisture content, chemical composition and sensory quality.[Results] The results showed that the pH value of tobacco leaf in the treatment group was higher than that in the control group, and such pH value remained stable with the storage time. The TSNAs levels in the treatment groups of Maryland and Burley tobacco were 40.4% and 36.2% less than that in the control group, respectively. There was no difference in appearance of tobacco leaves between the treatment group and the control group. The moisture content of the treatment group was higher than that of the control group. The total alkaloids and total sugar content in the treatment group were slightly lower than that in the control group. The ammonia and total volatile alkali in the treatment group were lower than that in the control group. The sensory quality of the treatment group was better than that of the control group.[Conclusion] It can be seen that sodium carbonate buffer can reduce the accumulation of TSNAs during storage of tobacco leaves, without negatively affecting the quality of tobacco leaves.
ZHANG Xinyu, CAO Fujun, OU Yafei, LIU Deshui, BAI Ruoshi, YAN Lihong, MA Yanjun, LIN Hui, ZHOU Jun
Comparison of quality of expanded cut burley tobacco with and without baking treatment
2020 Vol. 26 (3): 43-48 [Abstract] ( 5 ) HTML (0 KB)  PDF-CHN (1094 KB)  PDF-ENG  (0 KB)  ( 0 ) | Cite this article
Abstract：[Objective] Comparison of quality of expanded cut burley tobacco with and without baking treatment.[Method] Two production lines, flue-cured tobacco sheet process (without feeding) and burley tobacco sheet baking process (without feeding), were used separately to process single-type burley tobacco. After cutting, the burley tobacco shred was processed by the carbon dioxide cut tobacco expansion line with a hot air temperature of 290℃ before moisture regain process to produce expanded cut burley tobacco. The quality of expanded cut burley tobacco were evaluated to compare these two processes.[Results] (1) the silk rate and filling value of the unbaked burley tobacco was higher than that of the baked burley tobacco shred. (2) The total sugar content of the unbaked burley tobacco was higher than that of the baked burley tobacco. (3) The contents of NNN, NAT and NNK of the baked cut burley tobacco was lower than that of the baked burley tobacco. (4) The released amount of CO, NNK, NH3, phenol, and crotonaldehyde from the unbaked burley tobacco was lower than that of the baked burley tobacco; the hazard assessment index of the cigarette which made of unbaked burley tobacco was 10.07% lower than that made of baked burley tobacco. (5) The sensory quality score of the unbaked burley tobacco was higher than that of the baked burley tobacco.[Conclusion] For expanded cut burley tobacco production, no-baking process can not only simplify the process, but also help reducing the harm of cigarettes.
LU Yuhao, WANG Binbin, NIU Ruping, WAN Yunfei, ZHANG Juan, YANG Lei, YANG Ying
Effect of compaction position bias on cigarette processing quality
2020 Vol. 26 (3): 49-53,58 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1160 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
Abstract：Compaction position is the characteristic index of machining precision. The compaction position bias often affects the tobacco distribution and the cigarette quality. This paper carries out the compaction position bias gradient test and explores the effect of compaction position on cigarette physical indexes, smoke bears, and mainstream flue gas. Results showed that:(1) The compaction position bias led to the difference in the resistance, hardness, sensory quality, puff number, and tar amount of the inside and outside cigarette outlets, and such difference became increasingly significant with the increase of the compaction position bias. In particular, the difference was more significant after the position shift reached 3 mm. (2) The compaction position bias also led to an increase in the number of cigarettes with loose ends, and the number of cigarettes with decreased compaction ends reducing was larger than the number of cigarettes with increased compaction ends. (3) With the movement of the compaction position, the weight of the inside and outside cigarette with single compaction also differed, so there was a greater difference in the quality of the inside and outside cigarette with the single compaction than that with the double compaction. In order to improve the consistency of quality of the inside and outside cigarette, it was advisable to use a six-slot leveler, with the compaction position bias less than 3mm.
LING Xiaocun, JI Xiong, GONG Xiaojun, ZHOU Feng, HE Zhenkun, FANG Ren, LI Yuanzhang
Improvement of tipping quality of hard-shaped filter cigarettes
2020 Vol. 26 (3): 54-58 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (1422 KB)  PDF-ENG  (0 KB)  ( 0 ) | Cite this article
Abstract：[Objective] To solve the quality defects such as the dyeing of the end face of the filter, the burr of the tipping paper, and the horizontal stripes of the tipping paper when the hard-shaped filter tip is attached.[Method] Optimize the filter rod hardness, forming paper weight, tipping paper ink temperature resistance and adhesion fastness, reduce the width of the cutting drum wheel volume, increase the number of negative pressure suction holes, and install a filter cutter cleaning device.[Results] ① The end face of the filter was stained to 5 minutes after the curling before improvement to no longer completely stained; ② The proportion of burr cigarettes of tipping paper was reduced from 10.8% to 0.01‰; ③The proportion of horizontal cigarettes of tipping paper is reduced from 1.5% to 0.03‰.[Conclusion] This method can provide a reference for the optimization of the process of industrial hard modeling filter rods in production.
 AGRONOMY AND CURING
LIU Yong, HUANG Changjun, ZENG Jianmin, XIAO Binggang, YU Haiqin, LI Yongping
Breeding and characteristic of PVY resistant new variety Yunyan301 obtained by directional modification on basis of of Yunyan87 as femal parent
2020 Vol. 26 (3): 59-65 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (1337 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
Abstract：The PVY resistant " Yunyan301" is bred by the Yunnan Acedemy of Tobacco Agricultural Sciences, the National Tobacco Genetic Engineering Research Center. After cloning tobacco recessive PVY resistant gene eif4e1 (also known as the PVY susceptiable gene eIF4E1), developing functional molecular markers, and analyzing the domestic and foreign PVY resistant varieties and germplasma resources genotype, through continuous backcross, molecular marker-assisted selection Yunyan301 was bred by with Yunyan87 as the female parent and Y85 x RY2/F2 as male parent (carrying eif4e1) using whole genomic chip background detection (non-GMO). The genetic background of The Genetic background recovery rate from Yunyan301 to Yunyan87 was about 99.43%. Yunyan301 has significantly improved PVY resistance compared with Yunyan87, and retains the same cultivation and curing characteristics, original leaf smoke style characteristics and sensory quality as that of Yunyan87. In the Yunyan87 planting area where the PVY incidence rate reached 1% after topping, planting Yunyan301 can recover PVY-induced loss, which is a good choice withgood prospect of promotion.
XIAN Liguo, HUANG Yilan, WANG Songfeng, ZHANG Ying, ZHANG Ye, LIN Zhihui, LIU Hui, XU Xixiang, LIN Shuiliang, SUN Shuaishuai, SUN Fushan
Study on quality and curing characteristics of CB-1 fresh leaves
2020 Vol. 26 (3): 66-73 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (1454 KB)  PDF-ENG  (0 KB)  ( 5 ) | Cite this article
Abstract：In order to further explore the reason why characteristic tobacco variety CB-1 is difficult to be cured, this study investigates the differences in the SPAD, pigment, moisture, chemical composition, enzyme activity, organization structure and polyphenols between CB-1 and Yunyan 87 in mature fresh tobacco leaves as well as the color changing characteristics of leaves in the black-box. The results showed that there were significant differences in the quality and curing characteristics of mature fresh tobacco leaves of CB-1 and Yunyan 87. Compared with Yunyan87 mature fresh leaves, the average SPAD value of CB-1 mature fresh tobacco leaves was higher by 2.32, the chlorophyll and carotenoid contents were higher by 15.63% and 55.56%, respectively, the leaf bound moisture, total sugar and reducing sugar contents were higher, the activities of POD, PPO, SOD, amylase were higher, the thickness and palisade tissue of middle leaves were thicker by 3.07 μm, 8.60 μm, respectively, and the chlorogenic acid content was higher. CB-1 fresh leaves showed slower natural yellowing speed but faster browning speed. In fact, the leaves began to turn brown before turning almost completely yellow, indicating poor curability and curing resistance. This study provides a reference for regulating temperature and humidity, promoting yellowing and water loss coordination, reducing green-yellow, variegated and slick tobacco leaves of CB-1 flue curing.
MA Jing, LI Xueli, WANG Peng, SUN Shuguang, MA Junhong, PAN Haodong, YAO Pengwei, WANG Jing, YE Xiefeng
Effects of soil salinity on dry matter weight and absorption and distribution of mineral elements in flue-cured tobacco organs
2020 Vol. 26 (3): 74-83 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (1744 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
Abstract：In order to explore the effects of soil salinity on dry matter accumulation and mineral element content of flue-cured tobacco, a pot experiment was carried out. In this study, Zhongyan 100 was used as the test material, and the average salt concentration and ion composition of the cultivated layer of 20 sample sites in previous surveyed local tobacco field were used as the benchmark. The actual soil salt composition was simulated with CaCl2, Ca(HCO3)2, MgSO4, K2SO4, Na2SO4. The effects of 3, 6, 9 and 12 times of soil salinity on dry matter weight, mineral element content in different organs, Ca/Na, K/Na, Ca and K selective transport capacity of flue-cured tobacco were studied. During the same growth period, with the increase of salt concentration, the dry matter weight of various organs of flue-cured tobacco increased first and then decreased. Under 3-fold baseline concentration treatment, the dry matter weight was the highest and the K content in flue-cured tobacco leaves also increased; With the increase of salt concentration, the absorption of K, Ca, Cu, Fe, Mn elements in some periods was restricted. K/Na and Ca/Na in various organs of flue-cured tobacco decreased with the increase of salt concentration. Under high salt concentration, K/Na in different organs of flue-cured tobacco can be ranked as root > leaf > stem. High salt concentration promoted stem-to-leaf transportation of SK, Na and SCa,Na to a certain extent, and inhibited root-to-stem transportation of SK, Na and SCa, Na. Flue-cured tobacco has a certain tolerance to low concentration of salt, but under high salt concentration, the absorption-distribution balance of mineral nutrients is destroyed, which will negatively affect the growth and development of flue-cured tobacco.
 PLANT PROTECTION
CHANG Dong, GU Jianguo, JIA Fangfang, MA Wenhui, XU Yueqi, YAN Haitao
Screening and control efficacy of different plant-derived biocontrol bacteria against tobacco black shank disease
2020 Vol. 26 (3): 84-90 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (1680 KB)  PDF-ENG  (0 KB)  ( 3 ) | Cite this article
Abstract：Plant endophytes are valuable resources of biocontrol agents and play an important role in the biological control of crop diseases. In this paper, endophytic bacteria were isolated from 8 different plant stem tissues using the dilution plate method. Antagonistic bacteria were screened using the secondary plate pairing method. The bacterial species were identified through morphological observation, physiological and biochemical reactions, and 16S rDNA analysis. The potted plants and field tests were used to test the bacteria biocontrol and prevention effect, and measure its promoting effect. The results showed that a total of 89 strains of endophytic bacteria were isolated. Among them, 6 strains with better antagonistic effect on Phytophthora nicotianae were J11, J21 (paper mulberry), T3, T23 (potato), G3 (medlar) and KY (tobacco). KY was identified as Pseudomonas lini, J11, G3, and T3 were Bacillus cereus, T23 was Bacillus pumilus, and J21 was Bacillus subtilis. KY, T23 and J11 had better control effect against tobacco black shank disease, with the control efficiency of 75.41%, 63.93% and 70.49 in pot trials and 63.62%, 51.11% and 52.67% in field trials, respectively, and it can promote the growth of tobacco plant height and stem circumference.
LONG Ying, LIU Jianping, WU Shaolong, LI Xiaohong
Effects of different tobacco varieties on growth and food utilization efficiency of Spodoptera litura larvae
2020 Vol. 26 (3): 91-97 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (1503 KB)  PDF-ENG  (0 KB)  ( 0 ) | Cite this article
Abstract：This study aims to explore the effects of different tobacco varieties on the growth and food utilization efficiency of spodoptera litura larvae Six tobacco varieties were randomly selected as research objects in this study, including wild varieties of Changhua tobacco and Badan wild tobacco, local varieties of Liangqiao sun cured tobacco and Shuangguan sun cured tobacco, bred varieties of Xiangyan 5 and Zhongyan 100. The growth and nutritional response parameters of S.litura larvae fed on different varieties of tobacco were tested. Different varieties of tobacco all had significant effects on the dry weight, development duration, survival rate and various nutritional effect parameters of the larvae of S.litura. The dry weight, survival rate, relative growth rate, relative consumption rate, approximate digestibility and food utilization rate of the S.litura larvae were in the order of bred varieties > local varieties > wild varieties; the development duration was in the order of wild varieties > local varieties > bred varieties. It can be concluded that wild tobacco has higher resistance to S. litura than local and bred varieties.
 BIOTECHNOLOGY
HE Conglian, ZHENG Zhiyun, CHEN Yi, ZHAO Gaokun, HE Chenggang, HU Binbin, JIANG Yonglei, ZOU Congming
Screening differentially expressed proteins in response to senescence of tobacco leaves based on iTRAQ
2020 Vol. 26 (3): 98-107 [Abstract] ( 2 ) HTML (0 KB)  PDF-CHN (3030 KB)  PDF-ENG  (0 KB)  ( 1 ) | Cite this article
Abstract：[Objective] This study aims to reveal the mechanism of tobacco senescence from the protein level.[Methods] Flue-cured tobacco variety K326 was used as material, the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) at immature (IM) stage, well-mature (WM) stage and over-mature (OM) stage were measured. Isotope relative labeling and absolute quantitative technology (iTRAQ) were used to identify differentially expressed proteins in response to senescence of tobacco leaves at three stages. Bioinformatics analysis of the obtained differential proteins was performed to screen out potential target proteins for tobacco senescence. At the same time, 12 proteins were selected for parallel reaction monitoring (PRM) verification.[Results] (1) With the increase of tobacco leaves age, the activities of SOD, CAT, POD, and APX were gradually decreased. (2) A total of 4747 proteins were identified. In comparison of OM stage and IM stage, there were 321 differential expressed proteins, including 122 up-regulated proteins and 199 down-regulated proteins. In comparison of OM stage and WM stage, there were 319 differential expressed proteins, including 124 up-regulated ones and 195 down-regulated ones. In comparison of WM stage and IM stage, a total of 223 differential expressed proteins were found, including 125 up-regulated proteins and 98 down-regulated proteins. (3) According to GO functional annotations, differential expressed proteins are mainly involved in biological processes (metabolic processes and cellular processes), the cellular composition (cells, cell parts, and organelles) and molecular functions (catalytic activity). (4) As described in KEGG (Kyoto Encyclopedia of Genes and Genomes), differential expressed proteins are significantly enriched in metabolic pathways, photosynthesis, biosynthesis of secondary metabolites, and so on. (5) In the PRM verification, among the comparison groups of OM vs IM, OM vs WM and WM vs IM, there were 10/9/10 proteins meeting verification results, which was in line with the iTRAQ quantitative results, respectively. 6 differential expressed proteins including Q1WL43, A0A1S4DCM1, A0A1S4C433, A0A1S4AKK6, A0A1S3ZQP6 and A0A140G1P2 may be potential target proteins of tobacco senescence.[Conclusion] In this study, iTRAQ combined with PRM technology was used to screen differentially expressed proteins in tobacco leaves in response to senescence, which laid a solid foundation for in-depth understanding of the regulatory mechanism of response molecules in tobacco senescence.
DING Xiangyu, ZHAO Man, WANG Pengwei, DING Xinhua, CHU Zhaohui, ZHAO Xiangyu, LI Yang
Effect of over-expression of NtHQT on synthesis of caffeoyl quinic acids and flavonols in tobacco
2020 Vol. 26 (3): 108-113 [Abstract] ( 0 ) HTML (0 KB)  PDF-CHN (2285 KB)  PDF-ENG  (0 KB)  ( 2 ) | Cite this article
Abstract：[Background] Caffeoyl quinic acids (CQAs) and flavonols, two of important secondary metabolites of phenylpropanoids in plant, have been proved good for human health. However, there is only a minute amount of CQAs and flavonols in tobacco leaves.[Methods] NtHQT is an acyltransferase in tobacco that regulates the final step in the synthesis of CQAs. In order to investigate whether NtHQT has participated in synthesis of flavonols, we constructed the vetor pCXSN::NtHQT and introduced it into Nicotiana tabacum var. samsun.[Results] The concentrations of individual phenylpropanoids were increased to varying extents in NtHQT-overexpressing transgenic tobacco plants compared to wild-type plants. The concentration of CQAs was increased by 10.34-fold as maximum; and the concentrations rutin and kaempferol rutinoside were increased by 17.83- and 16.97-fold as maximum, respectively. However, the transgenic tobacco exhibited no significant differences from wild-type tobacco.[Conclusion] These results suggested NtHQT can positively regulate the synthesis of flavonols and CQAs, without affecting the growth and development of tobacco.
 ECONOMY AND MANAGEMENT
CHEN Changhua, HUANG He, YIN Jiankang, LIANG Yan, LI Maobo, HU Yin
Study on logistics benchmark classification system of tobacco business enterprises: A case study of Sichuan province
2020 Vol. 26 (3): 114-120 [Abstract] ( 3 ) HTML (0 KB)  PDF-CHN (1720 KB)  PDF-ENG  (0 KB)  ( 3 ) | Cite this article
Abstract：Aiming at the problems of incomparability of logistics benchmarking objects and poor usability of benchmarking results for tobacco commercial enterprises, this work proposes a method of constructing a logistics benchmarking classification system for tobacco commercial enterprises based on hierarchical classification. By fully considering the internal and external influencing factors and regional development imbalances, political, economic, social and technical factors are included in the classification index system, resulting in 12 initial classification indexes. Then grey correlation method and hierarchical clustering method are used to determine the weight of indicators and realize the classification. The empirical study shows that the 21 municipal tobacco logistics in Sichuan province can be divided into five categories, and verification was carried out based on total logistics costs. The difference in the total logistics costs between the categories is significant, while the difference in the total logistics costs in the same category is not, which indicates the accuracy of the classification results. This method can be applied to tobacco logistics benchmarking management at the three levels including industry, province and city, which provides an important basis for identifying benchmarking objects and improves the comparability and usability of logistics benchmarking objects.
LAI Baoqing, LIU Su, CHEN Huasheng
Design and implementation of standardized post authorization
2020 Vol. 26 (3): 121-126 [Abstract] ( 4 ) HTML (0 KB)  PDF-CHN (1469 KB)  PDF-ENG  (0 KB)  ( 3 ) | Cite this article
Abstract：According to the requirements of "The Tobacco Industry Informatization Development Plan (2014-2020)" and "Information Development Plan of Fujian Tobacco Business System in the 13th Five-year Plan Period", considering the current situation of information system authority management of tobacco commercial enterprises of Fujian province, a set of standardized post authorization management system is established. Through the standardization of post information and the application of master data in the downstream information system, the system authority is automatically associated with the personnel account, and is automatically recovered when the personnel leave the post. Review process of authority allocation of information system is established. Information system access control is strengthened and normalized.
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