Acta Tabacaria Sinica

2018 Vol. 24 No. 6 Published: 28 December 2018

MA Liyi, XU Zhiqiang, TIAN Zhenfeng, ZHAO Aixia, GAN Wuer

Determination of 7 high fatty acids in tobacco by LC-MS/MS

2018 Vol. 24 (6): 1-8 [Abstract] ( 8 ) HTML (0 KB) PDF-CHN (5443 KB) PDF-ENG (0 KB) ( 20 ) | Cite this article

FANG Zhengzhong, YANG Bin, CAI Guohua, BIAN Zhaoyang, LI Qiaoling, LIU Xiucai, ZHANG Dingfang, ZHANG Jianping

Determination of TiO₂ in cellulose acetate tow by continuous flow analysis

2018 Vol. 24 (6): 9-15 [Abstract] ( 10 ) HTML (0 KB) PDF-CHN (6028 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

GUO Weimin, GUO Jianhua, DONG Hongxu, XU Lei, HAN Jiasuo, LIU Wei, WANG Tao, ZHANG Shixiang, ZHANG Yanling, MOU Wenjun

Correlation between free amino acids and smoking quality of flue-cured tobacco and variations during flue-curing process

2018 Vol. 24 (6): 16-25 [Abstract] ( 18 ) HTML (0 KB) PDF-CHN (9301 KB) PDF-ENG (0 KB) ( 34 ) | Cite this article

HAN Donglin, SHAO Ning, LI Dongliang, GAO Yun, LUO Cheng, ZHU Xiaolan

Preparation of chitosan-ammonium polyphosphate flame retardant modified tobacco sheet and studies on combustion pyrolysis characteristics of the same

2018 Vol. 24 (6): 26-33 [Abstract] ( 14 ) HTML (0 KB) PDF-CHN (8173 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

ZHANG Yaping, ZHANG Xiaoyu, TIAN Zhong, PENG Xiaomeng, ZHOU Shun, WANG Xiaofeng, HE Qing, ZHENG Feng, SHE Shike, TIAN Zhenfeng, ZHU Dongliang

Evaluation of quality consistence of cigarette paper of different lots based on thermogravimetric analysis

2018 Vol. 24 (6): 34-41 [Abstract] ( 10 ) HTML (0 KB) PDF-CHN (6860 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

REN Mengjuan, SHI Hongzhi, DUN Songyang, YANG Huijuan, LI Hongliang

Changes in chemical composition in leaves of low-nicotine tobacco resulted from grafting of tobacco with eggplant

2018 Vol. 24 (6): 42-48 [Abstract] ( 17 ) HTML (0 KB) PDF-CHN (6459 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

ZHANG Xiaofan, LI Xueli, YAO Pengwei, LIU Xiaohan, XU Liang, JIAO Jinghua, SUN Kang, YE Xiefeng

Effects of complex salt treatment on physiological characteristics of flue-cured tobacco seedlings

2018 Vol. 24 (6): 49-57 [Abstract] ( 12 ) HTML (0 KB) PDF-CHN (8239 KB) PDF-ENG (0 KB) ( 17 ) | Cite this article

CHANG Dong, MA Wenhui, ZHANG Kai, WANG Xiaoqiang, HE Xiaobing, XU Yueqi, XU Fangda

Effect of biochar fertilizer on microbial functional diversity in tobacco growing soil

2018 Vol. 24 (6): 58-66 [Abstract] ( 8 ) HTML (0 KB) PDF-CHN (8574 KB) PDF-ENG (0 KB) ( 27 ) | Cite this article

LAI Ruiqiang, LI Ronghua, XIA Yanshi, GUO Peiguo, YUAN Qinghua, ZHAO Weicai

SSR marker-based genetic diversity analysis of tobacco germplasm and association analysis with resistance to bacterial wilt

2018 Vol. 24 (6): 67-77 [Abstract] ( 7 ) HTML (1 KB) PDF-CHN (1301 KB) PDF-ENG (0 KB) ( 8 ) | Cite this article

LIU Xiaowei, QIN Yuanxia, YUAN Lianlian, HE Qingyun, YANG Jinguang, SHEN Lili

Study on the bacteria effect of two PGPR biocontrol on TMV

2018 Vol. 24 (6): 78-85 [Abstract] ( 11 ) HTML (1 KB) PDF-CHN (1840 KB) PDF-ENG (0 KB) ( 8 ) | Cite this article

LIN Shifeng, WANG Rengang, YU Jing, REN Xueliang, ZHANG Jie, YU Shizhou

Cloning and expression analysis of a tobacco functional gene NtCNGC1 induced by Ralstonia solanacearum

2018 Vol. 24 (6): 86-92 [Abstract] ( 7 ) HTML (1 KB) PDF-CHN (1849 KB) PDF-ENG (0 KB) ( 8 ) | Cite this article

YANG Tao, LI Yanyan, ZHENG Lu, HUANG Junbin, HUANG Kai, GUO Li, ZHOU Peng, YUAN Yuebin, PENG Wuxing, LI Xihong

Analysis of genetic diversity of Alternaria longipes of tobacco in Hubei

2018 Vol. 24 (6): 93-99 [Abstract] ( 4 ) HTML (1 KB) PDF-CHN (1205 KB) PDF-ENG (0 KB) ( 11 ) | Cite this article

ZHANG Huimin

Configuration of full life cycle report management model of data center in Fujian Tobacco

2018 Vol. 24 (6): 100-105 [Abstract] ( 12 ) HTML (1 KB) PDF-CHN (1433 KB) PDF-ENG (0 KB) ( 16 ) | Cite this article

GAO Qian, GUAN Ying, MI Qili, HUANG Haitao, YANG Ji, XU Yuqiong, JIAN Xiaopeng, YAO Jianhua

Effects of oral use tobacco products on pathogenes of oral diseases

2018 Vol. 24 (6): 106-111 [Abstract] ( 23 ) HTML (1 KB) PDF-CHN (1054 KB) PDF-ENG (0 KB) ( 16 ) | Cite this article

JIN Yinan, XU Zicheng, ZHANG Huanwei, WANG Fazhan, CHEN Siang, XIONG Yanan, WEI Shuoguo

Research progress on salt stress of and salt-resistance-related genes in tobacco

2018 Vol. 24 (6): 112-118 [Abstract] ( 9 ) HTML (1 KB) PDF-CHN (954 KB) PDF-ENG (0 KB) ( 9 ) | Cite this article

CAI Xunhui, WANG Ruyi, FAN Yanjun, CHEN Tao, ZHOU Wei

Research progress of genomic analysis of Pseudomonas syringae pv. tabaci and differentiation of pathogenicity thereof

2018 Vol. 24 (6): 119-125 [Abstract] ( 8 ) HTML (1 KB) PDF-CHN (963 KB) PDF-ENG (0 KB) ( 17 ) | Cite this article

DAI Meiling, LI Qiang, XIE Yan, MAO Jianshu, WANG Jinjun, TAN Jun, ZHANG Yiyang, GAO Huafeng, YAN Chenbing, ZHANG Ruiqin, LIU Jiahong, ZHANG Jian, TAN Chen, YOU Xudong, ZHOU Jiheng

Study on the inhibitory effect of Eupatorium adenophorum extracts on Phytophthora parasitica var. nicotianae

2018 Vol. 24 (6): 126-133 [Abstract] ( 15 ) HTML (1 KB) PDF-CHN (1460 KB) PDF-ENG (0 KB) ( 10 ) | Cite this article

CHEN Qianqian, BIAN Chuanhong, Kang Yebin, ZHAO Shimin, LI Shujun

Isolation and identification of a strain of agricultural antagonistic actinomycete

2018 Vol. 24 (6): 134-139 [Abstract] ( 4 ) HTML (1 KB) PDF-CHN (1305 KB) PDF-ENG (0 KB) ( 14 ) | Cite this article

2018 Vol. 24 (6): 140-142 [Abstract] ( 4 ) HTML (1 KB) PDF-CHN (891 KB) PDF-ENG (0 KB) ( 3 ) | Cite this article

2018 Vol. 24 No. 6 Published: 28 December 2018

MA Liyi, XU Zhiqiang, TIAN Zhenfeng, ZHAO Aixia, GAN Wuer

Determination of 7 high fatty acids in tobacco by LC-MS/MS

2018 Vol. 24 (6): 1-8 [Abstract] ( 8 ) HTML (0 KB) PDF-CHN (5443 KB) PDF-ENG (0 KB) ( 20 ) | Cite this article

Abstract：A method for determining 7 high fatty acids in tobacco by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. Sample was saponified with KOH-methanol solution, extracted with dichloromethane, and then injected directly into sample for LC-MS/MS analysis using heptadecanoic acid as internal standard for quantitative analysis. Results showed that the linear regression lines for peak area ratios (analyte/internal standard) plotted against the concentration ratios (analyte/internal standard) fitted well (R2 ≥ 0.9818) for high fatty acids. The recoveries of standard addition ranged from 87.5% to 109% with relative standard deviation (RSD) of 1.1%~4.7%. The limit of detection was between 0.0004 and 0.007 mg/g, and that of quantification was between 0.001 and 0.02 mg/g. This method is simple, accurate and suitable for the determination of 7 high fatty acids in tobacco.

FANG Zhengzhong, YANG Bin, CAI Guohua, BIAN Zhaoyang, LI Qiaoling, LIU Xiucai, ZHANG Dingfang, ZHANG Jianping

Determination of TiO₂ in cellulose acetate tow by continuous flow analysis

2018 Vol. 24 (6): 9-15 [Abstract] ( 10 ) HTML (0 KB) PDF-CHN (6028 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

Abstract：In this study, the content of TiO2 in cellulose acetate tow for cigarette was determined by continuous flow analytical method (CFA). The method is based on chromogenic reaction of DAM and titanium(IV) which is contained in sample solution dissolved by microwave. Pipeline design of flow analyzer as well as factors such as mixing temperature, mixing time and acidity of mobile phase were investigated. Results showed that:(1) Under condition of room temperature, mixed reaction time of 3 min, and with hydrochloric acid (volume ratio of 0.8:1) as mobile phase, the redesigned flow line module of CFA could obtain more sensitive detection signals at 420nm wave length; (2) The LOD for titanium(IV) was 0.04μg/mL and LOQ was 0.14μg/mL with RSD ≤ 3%,and the recovery was within the range of 97.8%~101.5%; (3) Results by CFA method were similar to those obtained by method of YC/T 169.11-2009.

GUO Weimin, GUO Jianhua, DONG Hongxu, XU Lei, HAN Jiasuo, LIU Wei, WANG Tao, ZHANG Shixiang, ZHANG Yanling, MOU Wenjun

Correlation between free amino acids and smoking quality of flue-cured tobacco and variations during flue-curing process

2018 Vol. 24 (6): 16-25 [Abstract] ( 18 ) HTML (0 KB) PDF-CHN (9301 KB) PDF-ENG (0 KB) ( 34 ) | Cite this article

Abstract：In order to study correlation between free amino acids and smoking quality of flue-cured tobacco leaves and the variation rule of free amino acids during flue-curing process, 335 tobacco leaf samples of different quality characteristics from 6 typical flavor planting areas of China were prepared, and the relationship between concentration of free amino acids and smoking quality scores of flue-cured tobacco was investigated, and the variation of the concentration as well as composition of free amino acids during flue-curing process was analyzed. Results showed that:①There was a negative correlation between free amino acids concentration and smoking quality of flue-cured tobacco overall, especially for the basic amino acids, aromatic amino acids and γ-ABA, which presented a stable correlation law regardless of the planting area and different sampling year.②The majority of free amino acids concentration in tobacco leaves showed an upward and then downward trend in the curing process, and the relatively decline of basic, aromatic amino acids and γ-ABA during middle and late period of flue-curing was stronger than other kinds of amino acids. ③Combined with the results of smoking quality related analysis and flue-curing process variation, the strong and stable negative correlation rule between free amino acid and smoking quality of tobacco leaves could result from the Maillard reaction during flue-curing. The index of free amino acids has a good application prospect in the evaluation of flue-cured tobacco quality and targeted reduced content of free amino acids in flue-curing process is beneficial for the smoking quality improvement of flue-cured tobacco leaves.

HAN Donglin, SHAO Ning, LI Dongliang, GAO Yun, LUO Cheng, ZHU Xiaolan

Preparation of chitosan-ammonium polyphosphate flame retardant modified tobacco sheet and studies on combustion pyrolysis characteristics of the same

2018 Vol. 24 (6): 26-33 [Abstract] ( 14 ) HTML (0 KB) PDF-CHN (8173 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

Abstract：In this paper, tobacco flame retardant modified sheets were prepared using a reconstituted tobacco sheet (RTS) as substrate and chitosan (CS) and ammonium polyphosaphate (APP) as flame retardant materials by dry-powder and layer-by-layer coating techniques. The structure, combustion behaviour and thermal degradation of CS-APP-RTS were investigated by SEM, micro-scale combustion calorimetry (MCC) and thermogravimetric analysis. Result showed that the incorporation of CS and APP flame retardant film could greatly drive the thermal degradation process to shift from low temperature to high temperature, which therefore resulted in significant delay of degradation and a 23.7% increase of thermal residue at 850℃ compared with RTS. MCC experiments confirmed that the CS-APP-RTS with 5% CS and 3% APP exhibited a 50.7% reduction in peak heat release rate and a 35.8% reduction in total heat release, relative to the uncoated RTS. In addition, the maximum temperature of temperature distribution maps of cigarette made by CS-APP-RTS decreased by 77℃ during burning state, thus demonstrating the high reduction of temperature and flammability.

ZHANG Yaping, ZHANG Xiaoyu, TIAN Zhong, PENG Xiaomeng, ZHOU Shun, WANG Xiaofeng, HE Qing, ZHENG Feng, SHE Shike, TIAN Zhenfeng, ZHU Dongliang

Evaluation of quality consistence of cigarette paper of different lots based on thermogravimetric analysis

2018 Vol. 24 (6): 34-41 [Abstract] ( 10 ) HTML (0 KB) PDF-CHN (6860 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

Abstract：To accurately evaluate quality stability among different lots of cigarette paper, a method based on thermogravimetric analysis was established. The cigarette paper amount, thermal analysis heating rate, environmental gas and its flow rate were optimized by orthogonal test. The optimized results showed that sample amount was about 10mg, thermal analysis heating rate was 30℃/min, and air flow rate was 50mL/min. Under optimized condition, the control sample of cigarette paper was subjected to 5 parallel thermal analysis tests. Based on test results, similarity threshold of TG curve was defined, and used as the basis for judging quality stability of other batches of cigarette paper. Quality stability of cigarette paper of different batches could be effectively tested by this method. Results obtained were consistent with that from smoke component test. The proposed method is simple, accurate and precise, which is suitable for the evaluation of quality stability of cigarette paper of different batches.

REN Mengjuan, SHI Hongzhi, DUN Songyang, YANG Huijuan, LI Hongliang

Changes in chemical composition in leaves of low-nicotine tobacco resulted from grafting of tobacco with eggplant

2018 Vol. 24 (6): 42-48 [Abstract] ( 17 ) HTML (0 KB) PDF-CHN (6459 KB) PDF-ENG (0 KB) ( 12 ) | Cite this article

Abstract：In order to explore means of reducing nicotine content of tobacco leaves effectively by grafting technology, a pot experiment was carried out to study feasibility of producing very low nicotine content tobacco leaves and changes in chemical composition of low nicotine tobacco leaves obtained by grafting with eggplant (eggplant as rootstock, flue-cured variety Yunyan87 as scion). Three treatments were used, i.e. tobacco/tobacco, tobacco/eggplant without soil covering, tobacco/eggplant with soil covering. Results showed that grafting of tobacco with eggplant didn't cause significant change in botanical and agronomic characters of tobacco plant and leaf. Nicotine level of grafted tobacco leaves decreased dramatically, with nicotine content in fresh leaves lowered to 0.08%, and 0.10% in flue-cured leaves, which were 95% decrease compared with tobacco/tobacco control. Contents of nor-nicotine and anabasine were also decreased, although the percentage of decrease was less for anabasine, indicating that anabasine may have certain degree of biosynthesis capacity in leaves in addition to roots. Anatabine contents were not detectable in both fresh and cured leaves. Covering soil at the bottom of scion stimulated root production which was able to undergo alkaloid biosynthesis, resulting in a significant increase of nicotine content in grafted tobacco leaves compared with no soil covered grafted tobacco. Content of chlorophyll in tobacco/eggplant leaves was significantly higher and matured slower than control plants. Content of protein, starch and total nitrogen were higher, and total sugar and reducing sugar were lower. This study provides a new way to selectively reduce and regulate nicotine content in tobacco leaves through agricultural measures.

ZHANG Xiaofan, LI Xueli, YAO Pengwei, LIU Xiaohan, XU Liang, JIAO Jinghua, SUN Kang, YE Xiefeng

Effects of complex salt treatment on physiological characteristics of flue-cured tobacco seedlings

2018 Vol. 24 (6): 49-57 [Abstract] ( 12 ) HTML (0 KB) PDF-CHN (8239 KB) PDF-ENG (0 KB) ( 17 ) | Cite this article

Abstract：To investigate the physiological changes and internal regulation of flue-cured tobacco seedlings under complex salt treatment, Zhongyan100 was treated with 0, 0.3%, 0.6%, 0.9% and 1.2% saline and n (NaCl):n(Na2SO4)=1:1, changes of root system parameter, chlorophyll, cell membrane permeability, endogenous hormone and antioxidant enzyme activity were analyzed. Results showed that 1)Low concentration salt treatment could promote the tobacco seedlings in the short term, increasesynthesis of chlorophyll, improve efficiency of light utilization, and increase root length, surface area, volume and activity. 2) Prolonged treatment time and increase of concentration, tobacco seedlings showed a whole functional decline,;cell membrane, chlorophyll and root system were damaged, and the membrane lipid peroxidation was strong. 3) Under complex salt stress, endogenous hormones and antioxidant enzyme system reacted quickly. However, with prolonged treatment time, all mechanisms were in disorder. ABA and ZR showed an upward trend as a whole, ABA and ZR were on the rise. Except POD, SOD and CAT increased first and then decreased. It was concluded that low concentration of complex salt can promote growth of tobacco seedlings in the short term; long time and high concentration of salt will cause serious damage to tobacco seedlings.

CHANG Dong, MA Wenhui, ZHANG Kai, WANG Xiaoqiang, HE Xiaobing, XU Yueqi, XU Fangda

Effect of biochar fertilizer on microbial functional diversity in tobacco growing soil

2018 Vol. 24 (6): 58-66 [Abstract] ( 8 ) HTML (0 KB) PDF-CHN (8574 KB) PDF-ENG (0 KB) ( 27 ) | Cite this article

Abstract：To identify the effect of biochar fertilizer on soil abiotic and biotic properties and invalidate soil improvement with biochar fertilizer input, soil microbial activities and related processes in 0-20 cm soil layer were determined in a tobacco planting field after biochar fertilizer addition at rates of 0, 0.9, 1.5, and 2.1 t·hm-2. Results showed that biochar fertilizer decreased the content of mineral nitrogen while significantly increased the content of soil organic carbon (SOC), microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN), by the extent of 23~40%, 8~49% and 49~97% respectively, compared with control. Biochar fertilizer also increased average well colour development (AWCD) values in Biolog EcoPlates, Shannon-Wiener index (H), Simpson's dominance (D) and McIntosh diversity index (U), and decreased the evenness index (E). Principal component analysis and heatmap analysis clearly differentiated the treatments, and microbial use of six categories of substrates significantly increased after biochar fertilizer addition. This result indicated that the addition rate of 1.5 t·hm-2 biochar fertilizer could improve soil fertility and soil microbial activity.

LAI Ruiqiang, LI Ronghua, XIA Yanshi, GUO Peiguo, YUAN Qinghua, ZHAO Weicai

SSR marker-based genetic diversity analysis of tobacco germplasm and association analysis with resistance to bacterial wilt

2018 Vol. 24 (6): 67-77 [Abstract] ( 7 ) HTML (1 KB) PDF-CHN (1301 KB) PDF-ENG (0 KB) ( 8 ) | Cite this article

Abstract：Tobacco bacterial wilt (TBW) is one of the major diseases that affect tobacco yield and quality. The development of excellent alleles related to TBW resistance can accelerate the breeding process of resistant cultivar. In this study, 94 tobacco germplasm materials were selected as research objects, of which the genotype and population structure were analyzed by using 236 simple sequence repeat (SSR) markers. Association analysis based on linkage disequilibrium (LD) was performed for identification of molecular markers associated with TBW resistance. The results showed that 236 SSRs detected 904 alleles in 94 tobacco germplasm materials, with an average of 3.83 alleles per SSR. The polymorphism information content (PIC) values obtained by SSR markers ranged from 0.1361 to 0.8760, with an average of 0.5136. Based on the SSR genotyping data, 126 haplotypes were constructed. On this basis, the population structure was analyzed, with totally 94 germplasm materials being classified into 2 groups, indicating that the population structure was simple with relative narrow genetic background and could reduce false associations. Association analysis between haplotypes and TBW-resistant phenotyping data evaluated in two years of field trials showed that there were 7 haplotypes closely related to TBW resistance, and 3 of them showed close correlation with TBW resistance in both two years of field trials, with an averaged explanation rate over 10%. The results provided a reference for the marker-assisted selection of TBW-resistant materials in tobacco breeding program.

LIU Xiaowei, QIN Yuanxia, YUAN Lianlian, HE Qingyun, YANG Jinguang, SHEN Lili

Study on the bacteria effect of two PGPR biocontrol on TMV

2018 Vol. 24 (6): 78-85 [Abstract] ( 11 ) HTML (1 KB) PDF-CHN (1840 KB) PDF-ENG (0 KB) ( 8 ) | Cite this article

Abstract：Tobacco mosaic virus (TMV) is one of the main viruses that affect tobacco, with characteristics of wide host range, strong invasiveness and anti-adversity. Currently, there is few effective antiviral agent to control TMV. One feasible approach for TMV management is application of plant growth promoting rhizobacteria (PGPR), which could prevent plant from virus invading at the initial stage, as the PGPR could promote plant growth and inhibit virus directly. In this paper, two strains of PGPR, namely Sa and Sk, were identified, and the effect of growth-promoting and disease-preventing was studied. Results showed that Sa belonged to Bacillus sp. and Sk belonged to Pseudomonas sp.. Fermentation supernatants of both Sa and Sk had the effect of promoting tobacco growth and inactivating TMV in vitro. The inhibitory effects of crude protein, deproteinized supernatants and crude protein which was inactivated from Sa fermentation liquor on TMV were respectively 85.2%、83.1% and 36.2%, implying the bioactive substance of Sa was protein and non-protein element; the inhibitory effects of crude protein and deproteinized supernatants from Sa fermentation liquor on TMV were respectively 88.0% and 0%, suggesting the bioactive substance of Sk was mainly protein. By using TMV30B infectious clone tests, the passivation effect was more obvious in observation. The fermentation supernatant of both Sa and Sk could inhibit infection of TMV30B significantly. Spraying fermentation supernatant of Sa and Sk could prevent tobacco from TMV, with a respectivecontrol efficiency of 27.71% and 18.55%. In conclusion, strain Sa showed higher biocontrol effect than Sk, which indicated a huge potential for antiviral agents development.

LIN Shifeng, WANG Rengang, YU Jing, REN Xueliang, ZHANG Jie, YU Shizhou

Cloning and expression analysis of a tobacco functional gene NtCNGC1 induced by Ralstonia solanacearum

2018 Vol. 24 (6): 86-92 [Abstract] ( 7 ) HTML (1 KB) PDF-CHN (1849 KB) PDF-ENG (0 KB) ( 8 ) | Cite this article

Abstract：Previous comparative transcriptase revealed that a cyclic nucleotide-gated channel (CNGC) gene was up-regulated in tobacco bacterial wilt-resistant cultivar DB101 and may be involved in resistance to bacterial wilt in tobacco. In order to investigate its function in the defense responses of tobacco plants, the cDNA and genomic DNA sequences of this CNGC gene were isolated from DB101 by PCR and named as NtCNGC1. Sequence analysis indicated that the CDS length of NtCNGC1 was 2147 bop, encoding a protein of 717 amino acids with an average molecular weight of 83 kid, and a pig value of 9.35. Exon/intron structure analysis revealed 8 exons and 7 introns in NtCNGC1 genomic sequence. In addition, diverse cis-acting promoter elements involved in defense and stress responsiveness, pathogen responsiveness, salicylic acid responsiveness, fungal elicitor response, and wound-response were discovered in the promoter region of NtCNGC1. The expression pattern of NtCNGC1 in tobacco plants was assessed by qtr.-PCR. Tissue-specific expression analysis indicated that NtCNGC1 was mainly expressed in roots. When infected with Ralston solanacearum, the expression level of NtCNGC1 was significantly higher in resistant tobacco cultivar (DB101) than in susceptible tobacco cultivar (Honghuadajinyuan). In conclusion, NtCNGC1 proved to be a bacterial wilt resistance-related gene in tobacco.

YANG Tao, LI Yanyan, ZHENG Lu, HUANG Junbin, HUANG Kai, GUO Li, ZHOU Peng, YUAN Yuebin, PENG Wuxing, LI Xihong

Analysis of genetic diversity of Alternaria longipes of tobacco in Hubei

2018 Vol. 24 (6): 93-99 [Abstract] ( 4 ) HTML (1 KB) PDF-CHN (1205 KB) PDF-ENG (0 KB) ( 11 ) | Cite this article

Abstract：To reveal genetic characteristics of Alternaria longipes at molecular level, genetic diversity, genetic structure, differentiation and genetic variation of 34 strains of Alternaria longipes from four tobacco growing areas of Shiyan, Fuyang, Enshi and Yichang were analyzed by ISSR molecular marker method. Nei's diversity index and Shnanon's information index were ranged from 0.1118 to 0.2061 and 0.1972 to 0.3177 respectively, which indicated that there was abundant genetic diversity among A. longipes from different geographic areas. Genetic distance analysis showed that strains between Shiyan and Xiangyang and those between Enshi and Yichang were closely related. Population migration per generation (Nm) was 3.2740>1, indicating that there were gene flows among fungus from different geographic areas. According to UPGMA cluster analysis using NTSYS software, the 34 isolates can be classified into 4 groups when the similarity coefficient was set to 0.79. However, these strains can not be grouped according to geographical origin, indicating that there was no significant correlation between genetic diversity and geographical origin.

ZHANG Huimin

Configuration of full life cycle report management model of data center in Fujian Tobacco

2018 Vol. 24 (6): 100-105 [Abstract] ( 12 ) HTML (1 KB) PDF-CHN (1433 KB) PDF-ENG (0 KB) ( 16 ) | Cite this article

Abstract：In order to promote the application of data center in Fujian Tobacco and to improve the accuracy and utilization of report resource data, a full life cycle report management model of data center report was constructed based on many sectors of the Fujian Tobacco commercial enterprise data center platform, including marketing, monopoly, logistics, human capital, integrated plan, and so on. The lifecycle process of the report can be divided into incubation period, observation period, maturity stage and recession stage, including processes of requirements discovery, report preparation, report development, report operation, report management, and report abandonment. The proposed model is a complete PDCA management closed loop, which plays an important role in enhancing the efficiency of the whole data center project.

GAO Qian, GUAN Ying, MI Qili, HUANG Haitao, YANG Ji, XU Yuqiong, JIAN Xiaopeng, YAO Jianhua

Effects of oral use tobacco products on pathogenes of oral diseases

2018 Vol. 24 (6): 106-111 [Abstract] ( 23 ) HTML (1 KB) PDF-CHN (1054 KB) PDF-ENG (0 KB) ( 16 ) | Cite this article

Abstract：To explore the effect of oral smokeless tobacco (SLT) product on oral hygiene. Extracts of three different types of SLT, i.e. gel-based-type, bag-type, and flake-type, and traditional cigarette smoke total particulate matter (TPM) were formulated into different concentrations based on nicotine content, and antibacterial effect on Streptococcus mutans and Porphyromonas gingivalis was studied.Twenty volunteers were asked to take 3 types SLT or traditional cigarettes. Results were collected 5 minutes before intake and 0~2min, 2~5min, 5~10min, 10~15min, 90~95 min. after intake of tobacco. Bacterial DNA in saliva was extracted, and total bacteria, Streptococcus mutans and Porphyromonas gingivalis levels at each time point were determined by real-time fluorescent quantitative PCR (probe method). Results showed:(1) 3 types SLT had no significant effect on the numbers of Porphyromonas gingivali and Streptococcus mutans, but TPM had some inhibitory effect. (2) Total bacteria in saliva slightly decreased in the short-term after using SLT (gel-based-type and flake-type) and traditional cigarette, but after 1h both returned to pre-use levels. The change of Porphyromonas gingivalis and Streptococcus mutans was almost consistent with that of total bacteria. It was concluded that the use of SLT and traditional cigarettes will have a short-term inhibitory effect on oral microbiological content.

JIN Yinan, XU Zicheng, ZHANG Huanwei, WANG Fazhan, CHEN Siang, XIONG Yanan, WEI Shuoguo

Research progress on salt stress of and salt-resistance-related genes in tobacco

2018 Vol. 24 (6): 112-118 [Abstract] ( 9 ) HTML (1 KB) PDF-CHN (954 KB) PDF-ENG (0 KB) ( 9 ) | Cite this article

Abstract：Salt stress is a key research area in abiotic stress. Under salt stress, salt tolerance is enhanced in plants through stimulation of salt-resistance gene expression. This paper introduced the mechanism of tobacco salt stress. Research progress of salt resistance related genes in tobacco was reviewed from 5 aspects, including ion transport, osmoregulation, antioxidation, signal transduction and expression regulation, and protection of cells from stress damage. Future research direction of salt stress in tobacco was thus put forward.

CAI Xunhui, WANG Ruyi, FAN Yanjun, CHEN Tao, ZHOU Wei

Research progress of genomic analysis of Pseudomonas syringae pv. tabaci and differentiation of pathogenicity thereof

2018 Vol. 24 (6): 119-125 [Abstract] ( 8 ) HTML (1 KB) PDF-CHN (963 KB) PDF-ENG (0 KB) ( 17 ) | Cite this article

Abstract：Tobacco wildfire disease is a common bacterial disease in the world. To explore current progress on tobacco wildfire disease research, this paper summarized present research status of wildfire disease in tobacco based on Pseudomonas syringae pave. tabaci genomics and pathogenicity. Genomic features, pathogenicity island, type Ⅲ secretion system and virulence, horizontal gene transfer and adaptive evolution between pathogen and host of 5 Pseudomonas syringae strains were investigated. Pathogenic mechanism, physioloigical race of tobacco wildfire strain were analyzed. Suggestions were put forward for further research on tobacco wildfire disease.

DAI Meiling, LI Qiang, XIE Yan, MAO Jianshu, WANG Jinjun, TAN Jun, ZHANG Yiyang, GAO Huafeng, YAN Chenbing, ZHANG Ruiqin, LIU Jiahong, ZHANG Jian, TAN Chen, YOU Xudong, ZHOU Jiheng

Study on the inhibitory effect of Eupatorium adenophorum extracts on Phytophthora parasitica var. nicotianae

2018 Vol. 24 (6): 126-133 [Abstract] ( 15 ) HTML (1 KB) PDF-CHN (1460 KB) PDF-ENG (0 KB) ( 10 ) | Cite this article

Abstract：In order to evaluate the inhibitory effect of Eupatorium adenophorum extract on tobacco black shank, and provide a theoretical foundation for further development and application, effects of ethanol extract from three organs (leaves, stems and roots) of Eupatorium adenophorum on mycelial growth, sporangium yield and zoospore release rate of Phytophthora parasitica var. nicotianae were investigated using petri-dish. Results showed that, ethanol extracts of leaves, stems and roots on E. adenophorum could significantly inhibit growth of mycelium, sporangium yield and zoospore release rate, and the effect increased with enhanced treatment concentration. The median effective concentration (EC50) of ethanol extract of leaves,stems and roots on E. adenophorum was 7.74, 13.63 and 16.38 mg/mL, respectively. At the concentration of 10mg/mL, the inhibition rate of ethanol extracts from leaves, stems and roots on sporangium yield and zoospore release rate was above 50%. Ethanol extract of leaves, stems and roots all showed inhibitory effect on Phytophthora parasitica var. nicotianae, with extract of leaves being better than that of stems and roots.

CHEN Qianqian, BIAN Chuanhong, Kang Yebin, ZHAO Shimin, LI Shujun

Isolation and identification of a strain of agricultural antagonistic actinomycete

2018 Vol. 24 (6): 134-139 [Abstract] ( 4 ) HTML (1 KB) PDF-CHN (1305 KB) PDF-ENG (0 KB) ( 14 ) | Cite this article

Abstract：Experiment was carried out to isolate, screen and identify antagonistic actinomycetes in tobacco rhizosphere soil in Luoyang, so as to provide resources for further biological control of tobacco soil-borne diseases. Dilution plating method was adopted to isolate soil samples, antagonistic effects were determined by plate confrontation test method and mycelial growth rate method. Identification was carried out based on 16s rDNA analysis. A total of 108 actinomycetes colonies were isolated, which were obtained from tobacco rhizosphere soil samples through separation and purification. Among them, 22 strains had antagonistic effect against at least one of Phytophthora parasitica, Pythium aphanidermatum, and Fusarium oxysporum.The inhibition rate of SA74 on all 3 pathogenic fungus was more than 50%. According to morphological characteristics, physiological and biochemical activity, and molecular biology analysis, strain SA74 was identified as Streptomyces aureoverticillatus, and the classification status of the strain was Streptomyces aureoverticillatus.

2018 Vol. 24 (6): 140-142 [Abstract] ( 4 ) HTML (1 KB) PDF-CHN (891 KB) PDF-ENG (0 KB) ( 3 ) | Cite this article

Abstract：