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中国烟草学报
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中国烟草学报  2020, Vol. 26 Issue (4): 93-100    DOI: 10.16472/j.chinatobacco.2020.104
  农艺与调制 本期目录 | 过刊浏览 | 高级检索 |
赤星病烤后烟叶内生及叶际真菌分析
向立刚1,2, 汪汉成2, 郑苹2, 蔡刘体2, 余知和1
1 长江大学生命科学学院, 荆州 434025;
2 贵州省烟草科学研究院, 贵阳 550081
Analysis of endophytic fungi and phyllosphere fungi of flue cured tobacco leaves with brown spot disease
XIANG Ligang1,2, WANG Hancheng2, ZHENG Ping2, CAI Liuti2, YU Zhihe1
1 Life Science College of Yangtze University, Jingzhou Hubei 434025, China;
2 Guizhou Academy of Tobacco Science, Guiyang Guizhou 550081, China
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摘要 [背景] 由Alternaria alternata引起的烟草赤星病是烟叶成熟期主要真菌病害,在大田期和烘烤期均会造成严重危害,该病害的发生与叶片内生及叶际真菌群落密切相关。[方法] 本文以健康与感赤星病烤后烟叶为研究对象,通过PCR技术扩增真菌18S rRNA转录间隔区的ITS1区,采用Illumina Miseq高通量测序技术分析赤星病烤后烟叶内生及叶际真菌群落结构与多样性。[结果] 链格孢属Alternaria为赤星病烤后烟叶的优势菌属,相对丰度>70%;其次为红酵母属Rhodotorula,相对丰度>10%,其余真菌属相对丰度均小于1%。链格孢属和红酵母属均为健康烤后烟叶的优势真菌,但二者的相对丰度之和小于赤星病烤后烟叶;健康烤后烟叶中相对丰度>1%的菌属还包括曲霉属AspergillusWallemia等。Alpha多样性结果表明,赤星病烤后烟叶真菌群落丰富度和多样性指数均低于健康烤后烟叶。[结论] 赤星病烤后烟叶内生及叶际真菌群落多样性低于健康烤后烟叶,其优势菌属为链格孢属和红酵母属。
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向立刚
汪汉成
郑苹
蔡刘体
余知和
关键词:  烟草赤星病  链格孢属  内生真菌  叶际真菌  Illumina高通量测序    
Abstract: Flue-cured tobacco brown spot disease is a main fungal disease in the ripening stage of tobacco leaves caused by Alternaria alternata, which can cause huge economic loss in the field period and baking period. Endophytic and phyllosphere fungi are closely related to the happening of such disease. In this study, the PCR technology was used to amplify ITS1 region of all the fungal transcribed spacer regions of the flue-cured leaves of healthy and diseased plants, and then the leaf fungal community structure and diversity were analyzed using Illumina Miseq high-throughput sequencing technology. The result showed that Alternaria was dominant genus in all diseased tobacco leaves, and the relative abundance of Alternaria in each sample was more than 70%. Rhodotorula was second dominant genus in all diseased tobacco leaves, and the relative abundance in each sample was more than 10%. The relative abundances of other genera of fungi were less than 1% in all diseased leaves. For healthy tobacco leaves, Alternaria and Rhodotorula were the dominant genera, the sum of relative abundances of the two was smaller than that in diseased tobacco leaves. In addition to the above two species, the relative abundance of Aspergillus, Wallemia and Unclassified_o_Pleosporales, etc., were also greater than 1%. The results of alpha diversity analysis showed that the richness and diversity index of the fungal community in the diseased leaves were lower than that in the healthy leaves. Alternaria and Rhodotorula were the dominant genera in both healthy and diseased flue-cured tobacco leaves.
Key words:  tobacco brown spot diease    Alternaria    endophytic fungi    phyllosphere fungi    Illumina high-throughput sequencing
收稿日期:  2020-04-17                     发布日期:  2020-09-18      期的出版日期:  2020-09-18
基金资助: 国家自然科学基金(31960550);中国烟草总公司科技项目(110202001035(LS-04));贵州省科技厅优秀青年人才培养计划(黔科合平台人才[2017]5619);中国烟草总公司贵州省公司科技项目(201714,201914,2020XM22)
通讯作者:  汪汉成(1982-),Tel:18984335592,Email:xiaobaiyang126@hotmail.com;余知和(1966-),Tel:13972385462,Email:zhiheyu@hotmail.com    E-mail:  xiaobaiyang126@hotmail.com;zhiheyu@hotmail.com
作者简介:  向立刚(1994-),硕士研究生,主要从事烟草病害及生物信息学研究,Tel:17685026147,Email:1475206901@qq.com
引用本文:    
向立刚, 汪汉成, 郑苹, 蔡刘体, 余知和. 赤星病烤后烟叶内生及叶际真菌分析[J]. 中国烟草学报, 2020, 26(4): 93-100.
XIANG Ligang, WANG Hancheng, ZHENG Ping, CAI Liuti, YU Zhihe. Analysis of endophytic fungi and phyllosphere fungi of flue cured tobacco leaves with brown spot disease. Acta Tabacaria Sinica, 2020, 26(4): 93-100.
链接本文:  
http://ycxb.tobacco.org.cn/CN/10.16472/j.chinatobacco.2020.104  或          http://ycxb.tobacco.org.cn/CN/Y2020/V26/I4/93
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